Modifying Enzyme

Toyobo has various modifying enzymes. The detailed information can be referred in the following linked sites.

Applicatins Product Name
High efficient reverse transcription ReverTra Ace®
Stable transcription (T7 promoter) Thermo T7 RNA polymerase
Substrate for reverse transcription dNTPs Mixture
Dephosphorylation of DNA fragments E. coli Alkaline Phosphatase
Dephosphorylation of DNA fragments Calf intestine Alkaline Phosphatase
Kination of DNA fragments T4 Polynucleotide Kinase

ReverTra Ace® High Efficient Reverse Transcriptase

  • RNase minus M-MLV RTase with improved performance
  • Enables the synthesis of longer cDNAs (≥ 14 kb) than the WT-enzyme
  • Exhibits excellent reaction efficiency at high temperatures.

ReverTra Ace® is a high efficient M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase that has been genetically modified to remove RNase H activity and increase reaction efficiency. It is the preferred enzyme for applications requiring full-length cDNAs and high product yields from total RNA, mRNA, rRNA, etc.

  • Code No. TRT-101 10,000U

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Thermo T7 RNA polymerase Thermostable T7 RNA polymerase

  • Exhibits greater specific activity than WT-enzyme at 37-50ºC.

Thermo T7 RNA polymerase is a genetically modified T7 RNA polymerase exhibiting increased thermal stability. The optimum reaction temperature of this enzyme is around 50ºC. The half-life of the enzyme at 50ºC is approximately 85 min.

  • Code No. TRL-201 7,500U

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dNTPs Mixture(2mM) dNTPs Mixture(10mM)

  • 2 mM and 10 mM solutions are available
  • Suitable for PCR and reverse transcriptation

dNTPs Mixture is an equal moler solutoin mixture of ultrapure dATP, dCTP, dGTP and dTTP.

  • Code No. NTP-201 2 µmoles/1 ml (2 mM)
  • Code No. NTP-301 2 µmoles/0.2 ml (10 mM)

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E. coli Alkaline Phosphatase

  • Suitable for reactions at high temperatures due to its high heat stability.

E. Coli alkaline phosphatase catalyzes the removal of 5’-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. It can be used to prevent self-ligation of vectors because alkaline phosphate-treated DNA fragments lack the 5’-phosphoryl termini required for the actions of DNA ligases.

  • Code No. BAP-111 100U

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Calf Intestine Alkaline Phosphatase

  • Can be inactivated by heating at 65ºC for 30 min.

Calf intestine alkaline phosphatase catalyzes the removal of 5’-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. It can be used to prevent self-ligation of vectors because alkaline phosphate-treated DNA fragments lack the 5’-phosphoryl termini required by DNA ligases.

  • Code No. CAP-101 1,000U

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T4 Polynucleotide Kinase

  • Two different reaction buffers for different 5’-ends of nucleic acids are included in the kit.
  • Highly purified enzyme from a recombinant source

T4 polynucleotide kinase catalyzes the transfer and exchange of phosphate groups from the γ-position of ATP to the 5’–hydroxyl terminus of nucleic acids (double and single-stranded DNA and RNA). It can be used for the phosphorylation of DNA fragments or PCR primers. The efficiency of the reaction is affected by the status of the ends of the DNA strand (i.e., 3’-overhang, 5’-overhang, blunt or single strand).

  • Code No. PNK-111 1,500U

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