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Modifying Enzyme

Toyobo has various modifying enzymes. The detailed information can be referred in the following linked sites.

Applicatins Product Name
High efficient reverse transcription ReverTra Ace®
Stable transcription (T7 promoter) Thermo T7 RNA polymerase
Substrate for reverse transcription dNTPs Mixture
Dephosphorylation of DNA fragments Bacterial Alkaline Phosphatase
Dephosphorylation of DNA fragments Calf intestine Alkaline Phosphatase
Kination of DNA fragments T4 Polynucleotide Kinase

High Efficient Reverse Transcriptase

ReverTra Ace®

(click here)

  • RNase minus M-MLV RTase with improved performance
  • Enables the synthesis of longer cDNAs (≥ 14 kb) than the WT-enzyme
  • Exhibits excellent reaction efficiency at high temperatures.

Code No. TRT-101 10,000U


ReverTra Ace® is a high efficient M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase that has been genetically modified to remove RNase H activity and increase reaction efficiency. It is the preferred enzyme for applications requiring full-length cDNAs and high product yields from total RNA, mRNA, rRNA, etc.

Thermostable T7 RNA polymerase

Thermo T7 RNA polymerase

(click here)

  • Exhibits greater specific activity than WT-enzyme at 37-50ºC.

Code No. TRL-201 7,500U


Thermo T7 RNA polymerase is a genetically modified T7 RNA polymerase exhibiting increased thermal stability. The optimum reaction temperature of this enzyme is around 50ºC. The half-life of the enzyme at 50ºC is approximately 85 min.

dNTPs Mixture(2mM)

dNTPs Mixture(10mM)

(click here)

  • 2 mM and 10 mM solutions are available
  • Suitable for PCR and reverse transcriptation

Code No. NTP-201 2 µmoles/1 ml (2 mM)

Code No. NTP-301 2 µmoles/0.2 ml (10 mM)


dNTPs Mixture is an equal moler solutoin mixture of ultrapure dATP, dCTP, dGTP and dTTP.

E. coli Alkaline Phosphatase

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  • Suitable for reactions at high temperatures due to its high heat stability.

Code No. BAP-111 100U


E. Coli alkaline phosphatase catalyzes the removal of 5’-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. It can be used to prevent self-ligation of vectors because alkaline phosphate-treated DNA fragments lack the 5’-phosphoryl termini required for the actions of DNA ligases.

Calf Intestine Alkaline Phosphatase

(click here)

  • Can be inactivated by heating at 65ºC for 30 min.

Code No. CAP-101 1,000U


Calf intestine alkaline phosphatase catalyzes the removal of 5’-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. It can be used to prevent self-ligation of vectors because alkaline phosphate-treated DNA fragments lack the 5’-phosphoryl termini required by DNA ligases.

T4 Polynucleotide Kinase

(click here)

  • Two different reaction buffers for different 5’-ends of nucleic acids are included in the kit.
  • Highly purified enzyme from a recombinant source

Code No. PNK-111 1,500U


T4 polynucleotide kinase catalyzes the transfer and exchange of phosphate groups from the γ-position of ATP to the 5’–hydroxyl terminus of nucleic acids (double and single-stranded DNA and RNA). It can be used for the phosphorylation of DNA fragments or PCR primers. The efficiency of the reaction is affected by the status of the ends of the DNA strand (i.e., 3’-overhang, 5’-overhang, blunt or single strand).