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High Efficient Reverse Transcriptase: ReverTra Ace®


ReverTra Ace® is an M-MLV reverse transcriptase that has been improved by point mutation technology. ReverTra Ace® has two mutations in the polymerase and RNase H domains that elevate the cDNA synthesis ability and inactivate RNase H activity, respectively.Therefore, ReverTra Ace® (Code No. TRT-101) exhibits efficient reverse transcriptase activity and enables the synthesis of ≥ 14 kb cDNA.


ReverTra Ace-α-® (Code No.FSK-101) is an efficient and convenient kit for synthesizing high quality cDNA. The kit contains the highly efficient reverse transcriptase "ReverTra Ace®" as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR.

The ReverTra Ace® qPCR RT Kit (Code No. FSQ-101) is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR.



Fig.1 Point mutation in M-MLV RTase




A : ReverTra Ace


B : Reverse Transcriptase (Company A)


C : Reverse Transcriptase (Company B)

Fig.2 Comparison of reverse transcriptase efficiency.

Reverse transcriptase efficiency among various RTases is compared by RT-PCR of 18S rRNA. The RTase reaction was performed using a specific primer at 42ºC.