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GenNextTM Transposase-based DNA Library Prep Kit
Code No.TNP-101T, TNP-101, TNP-101L
*This product is not available in the U.S. and Europe.
DESCRIPTION
The GenNext™ Transposase-based DNA Library Prep Kit is designed for the simple and rapid preparation of next-generation sequencing (NGS) libraries from small amounts of double-stranded DNA.
This kit also supports the construction of NGS libraries suitable for full-length total RNA sequencing and allows for strand-specific analyses.
This kit utilizes transposase-mediated fragmentation and tagmentation, eliminating the need for pre-fragmentation of the input DNA. Additionally, the adoption of a proprietary library preparation method ensures high efficiency, simplicity, and speed.
When combined with a kit for synthesizing double-stranded DNA from RNA, this kit also enables the preparation of NGS libraries from small amounts of RNA.
Features
- Enables Library Preparation from Small Amounts of DNA
Compatible with 100 pg to 10 ng of total RNA as input (1 ng recommended).
- Low-bias amplification
A proprietary PCR enzyme (UKOD Polymerase) ensure efficient amplification of both AT-rich and GC-rich targets, enabling low-bias library amplification.
- Enables Library Preparation from Non-Fragmented DNA
Utilizes a transposase-mediated fragmentation and tagmentation reaction, allowing the use of non-fragmented DNA as input.
- Simple, Fast, and Highly Efficient
Employs a proprietary library preparation method that enables high-efficiency library construction in under 2 hours.
- Compatible with RNA-to-DNA Conversion Kits
Can be used in combination with kits for synthesizing double-stranded DNA from RNA, such as:
GenNext™ RamDA-seq™ Single Cell Kit (Code: RMD-101, RMD-101T)
GenNext™ Shin-RamDA-seq™ Single Cell Stranded Kit w/o LP (Code: RML-111, RML-111T)
Fortissimo™ GenNext™ Shin-RamDA-seq™ Kit for Degraded RNA (Code: RMF-111, RMF-111T)
These combinations enable library preparation from small amounts of RNA. Please refer to the respective instruction manuals when using these kits together.
Details
STORAGE CONDITION
Store at -20°C
COMPONENTS
The kits include the following reagents, which can be used for 8 (TNP-101T), 24 (TNP-101) and 96 (TNP-101L) reactions. All reagents should be stored at -20°C.
| GenNext™ Transposase-based DNA Library Prep Kit | TNP-101T | TNP-101 | TNP-101L |
|---|---|---|---|
| Size | 12 Rxns | 48 Rxns | 96 Rxns |
| Tagmentation Buffer | 37.5 μL | 150 μL | 300 μL |
| Transposase for Tagmentation | 30 μL | 120 μL | 240 μL |
| Additional Solution | 30 μL | 120 μL | 240 μl |
| Gap Repair Buffer | 156 μL | 624 μL | 1248 μl |
| Gap Repair Enzyme Mix | 54 μL | 216 μL | 432 μL |
| KOD Master Mix | 120 μL | 480 μL | 960 μL |
*Adapters and magnetic beads for purification are not included in this product.
Workflow
*1 When this kit is used for whole-genome sequencing (WGS), DNA samples with genome sizes of several megabase pairs (Mbp) or less, such as microbial genomes, are recommended.
Application Data
Example 1.Comparative Library Yield
Libraries were prepared from 1 ng of E. coli genomic DNA using a GenNext™ Transposase-based DNA Library Prep Kit and a competitor’s transposase-based kit. Twelve cycles of PCR were performed, followed by sequencing on an Illumina MiSeq™ system.
Library yields comparable to those obtained with the competitor’s kit were achieved, in all cases > 4 nM, permitting sequencing onIllumina platforms.
Example 2.Comparison of Library Coverage
Libraries were prepared from 1 ng of Thermus thermophilus HB8 (Tth) genomic DNA using a GenNext™ Transposase-based DNA Library Prep Kit and a competitor’s transposase-based library preparation kit. Coverage across different GC content ranges was evaluated. Twelve cycles of PCR were performed, followed by sequencing on an Illumina MiSeq™ instrument.
Libraries prepared with a GenNext™ kit exhibited normalized coverage values close to 1 spanning a range of GC contents, indicating significantly reduced GC bias compared with libraries prepared using the competitor’s kit.
