Recovering exosomes with high yields
through straightforward procedures

CATAROSEV CATAROSEV

What are exosomes?About

Exosomes are a type of extracellular vesicle, typically about 100 nanometers in diameter, enclosed by a lipid bilayer membrane and secreted by cells. They are present in various bodily fluids, such as blood and urine, and circulate throughout the body.
Exosomes are rich in biologically active substances and are increasingly recognized as crucial messengers in intercellular communication.
There are high expectations for their application in diagnosing and treating a wide range of diseases, including cancer, as well as in regenerative medicine, driving intensive research worldwide and attracting significant attention.

What Makes Our Exosome Recovery Technology Outstanding!Refining technology

  • Exosome recovery steps

    Without cumbersome pretreatment, our technology enables purification and recovery of exosomes in approximately 30 minutes* through three simple steps: adsorption, washing, and elution.
    *The time required for recovery process from 5ml of culture supernatant (HEK293.2sus).

    Toyobo’s CATAROSEV™ exosome recovery kit purifies and recovers exosomes through a straightforward workflow of adsorption, washing, and elution—steps that involve solution permeation.

    CATAROSEV™ is a separation membrane with positively charged pores approximately 200 nanometers in diameter. In the adsorption step, it selectively captures fine particles such as exosomes and microvesicles by exploiting the electrostatic attraction between negatively charged exosomes and the positively charged membrane. Through this process, most impurities, such as proteins in biological samples, are removed.

    In the washing step, any remaining impurities are removed using a washing solution.

    Finally, in the elution step, high-purity exosomes are recovered by selectively eluting the adsorbed exosomes with a salt-containing elution solution.

  • Comparison of CATAROSEV™
    with other exosome recovery methods

    * These comparisons are based on our company’s own studies under specific conditions and do not guarantee the performance or other characteristics of each method.

    POINT

    CATAROSEV™, a novel technique developed by Toyobo, requires a shorter working time and offers superior operability and yield compared with conventional methods.
    It can also selectively recover particles 50 to 150 nanometers in diameter, which are rich in exosomes.

  • Comparison of exosome yield

    Compared with other methods, CATAROSEV™ delivers superior exosome yield, an advantage expected to shorten processing times and improve the efficiency of exosome purification for research and development.

    * These values are based on our internal test data and are not guaranteed.

    POINT

    The exosome yield achieved by Toyobo’s novel technique is approximately 50% higher than that of the ultracentrifugation method and more than 10 times higher than that of a competing method.

  • Comparison of exosome particle diameters

    Compared with the ultracentrifugation method, CATAROSEV™ selectively recovers particles 50 to 150 nanometers in diameter, which are rich in exosomes.

    • Particle analysis by NanoSight

      Exosomes recovered from cell culture supernatants by the ultracentrifugation method and by the novel CATAROSEV™ technique were each diluted to an appropriate concentration with ultrapure water. Particle diameter and concentration were then analyzed using a NanoSight NS300.

      What is NanoSight?

      “NanoSight is an analytical instrument that uses Nano Tracking Analysis (NTA) to visualize the Brownian motion of nanoparticles suspended in a solvent, enabling measurement of particle size and particle concentration. Even when the suspension contains a mixture of various substances or a polydisperse distribution of particle sizes, the Brownian motion captured by the visualization technology allows determination of particle counts for each particle-size population.”

      CATAROSEV™

      Ultracentrifugation method

      * These values are based on our internal test data and are not guaranteed.

      POINT

      • Toyobo’s novel technique fractionates particles within the typical exosome diameter range (50–150 nanometers).
      • Compared with the centrifugation method, exosome purity is higher with CATAROSEV™

    • Comparison of recovered exosome amounts by Western blotting

      Samples recovered from HEK293 cell culture supernatant using ultracentrifugation and the new technology (CATAROSEV™) were electrophoresed and subsequently analyzed by Western blotting using anti-Tsg101, anti-CD63, and anti-CD9 antibodies.

      POINT

      Larger amounts of exosomes were recovered with the CATAROSEV™ technique than with the ultracentrifugation method.

Easy-to-understand video
explanation of new technologyMovie

  • Special interview on exosome recovery technology

    Project Associate Professor Naohiro Seo, a co-developer of this technology, explains its key features, development background, and future prospects.

Exosome applicationsPurpose

  • Exome research

  • Regenerative medicine

  • Cosmetics

  • Pharmaceuticals

Frequently asked questionsFAQ

  • About the Technology

    • What is the newly developed purification method that enables simple and high‑yield exosome recovery?
      It fractionates particles based on exosome size and electric charge.
    • How does the newly developed recovery technology compare with the ultracentrifugation method in exosome yield?
      The exosome yield using CATAROSEV™ is approximately 50% higher than that of the ultracentrifugation method.
    • How does the newly developed recovery technology compare with the magnetic bead method in exosome yield?
      The exosome yield using CATAROSEV™ is approximately 10 times higher than that of the magnetic bead method.
    • What are the advantages of using the novel CATAROSEV™ recovery method over conventional methods?
      CATAROSEV™ enables easier fractionation of particles 50–150 nanometers in diameter—the size range with high-purity exosome content—than ultracentrifugation. It is also capable of capturing a greater number of particles.
    • How long does it take to recover exosomes using CATAROSEV™?
      CATAROSEV™ can purify and recover exosomes in approximately 30 minutes.

  • About the recovery kit

    • What is CATAROSEV™?
      * CATAROSEV™ is a newly developed exosome recovery kit from Toyobo.
      CATAROSEV™ is designed to isolate and purify exosomes in three simple steps, enabling high-yield recovery.
    • How high is the exosome yield when using CATAROSEV™?
      The exosome yield with CATAROSEV™ is about 50% higher than with ultracentrifugation and more than 10 times higher than with the magnetic bead method.
    • How high is the exome particle concentration when using CATAROSEV™?
      For the exosome yield comparison, particle size and concentration were analyzed using a NanoSight NS300.
      Click here for the results.
    • How can the exosomes recovered with CATAROSEV™ be confirmed?
      Exosome yield can be confirmed using a NanoSight, which measures nanoparticle size and concentration, as well as by Western blotting and ELISA.
    • How many times can CATAROSEV™ be used per unit?
      Each CATAROSEV™ kit can be used to recover exosomes from a single sample. For details, please refer to the manual.
    • Does CATAROSEV™ require any special equipment?
      No special equipment is required, and no prior preparation is necessary.
    • Is any support provided on how to use CATAROSEV™?
      We offer support on how to use the kit and troubleshooting, so please feel free to contact us.
    • もっと見る