cDNA synthesis kits

Toyobo has various kits for cDNA synthesis based on the genetically improved M-MLV reverse transcriptase "ReverTra Ace®". ReverTra Ace -α-® is an efficient and convenient kit to synthesize high quality cDNA. This kit contains the highly efficient reverse transcriptase "ReverTra Ace®", as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR.
ReverTra Ace® qPCR RT Kit (Code No. FSQ-101) is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR. This kit contains the highly efficient reverse transcriptase "ReverTra Ace®" and a RT buffer optimized for the highly efficient synthesis of short-chain cDNAs suitable for real-time PCR. ReverTra Ace® qPCR Master Mix (Code No. FSQ-201) is a premix version of the ReverTra Ace® qPCR RT Kit.
ReverTra Ace® qPCR RT Master Mix with gDNA remover (Code No. FSQ-301) is an efficient and convenient kit, consisting of master mix reagents, to synthesize high quality cDNAs for real-time PCR. The kit includes reagents for reverse transcription and for the removal of genomic DNA. The protocol consists of i) a genomic DNA degradation step using "gDNA remover [DNase I]" and ii) a reverse transcription step. The two steps can be achieved sequentially without purification or heat inactivation of DNase I.

cDNA synthesis Kits

Enzyme Product Name Application Efficiency Long cDNA
synthesis
Master Mix Genomic DNA
removal
Recommend PCR enzymes
ReverTra Ace® ReverTra Ace -α-® Conventional
RT-PCR
+ ++
(> 10 kb)
    KOD –Plus- series
KOD FX series
Taq-based PCR reagent
ReverTra Ace® qPCR RT Kit qPCR ++ +
(< 5 kb)
    THUNDERBIRD® series
Realtime PCR Master Mix series
KOD SYBR® qPCR Mix
KOD –Plus- series
KOD FX series
Taq-based PCR reagent
ReverTra Ace® qPCR RT Master Mix qPCR ++ +
(< 5 kb)
 
ReverTra Ace® qPCR RT Master Mix with gDNA Remover qPCR ++ +
(< 5 kb)
SuperPrep® Cell Lysis & RT Kit for qPCR qPCR from
cultured cells
++ +
(< 5 kb)
SuperPrep® II Cell Lysis & RT Kit for qPCR qPCR from cultured cells +++ +
(< 5 kb)

++: Excellent, +: Good, ✓: Applicable

ReverTra Ace -α-® High Efficient Reverse Transcription Kit

  • This kit contains all components for reverse transcription.
  • This kit enables the synthesis of ≥ 14 kb cDNA.

ReverTra Ace-α-® is an efficient and convenient kit to synthesize high quality cDNA. This kit contains the highly efficient reverse transcriptase "ReverTra Ace®", as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR. ReverTra Ace® is an M-MLV reverse transcriptase that has been improved by point mutation technology. ReverTra Ace® has two mutations in the polymerase and RNase H domains.

  • Code No. FSK-101 100 reactions

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ReverTra Ace® qPCR RT Kit & Master Mix
High Efficient Reverse Transcription Kit for Real-time PCR

  • The optimized RT buffer and Primer Mix (oligo dT & random primers) enable the highly efficient reverse.
  • The reaction can be completed in 15 min. The protocol does not contain an additional RNase H treatment step to remove residual RNA after reverse transcription.(Patent Pending)
  • Since the RT buffer is optimized for real-time PCR, the addition of 20% (v/v) of the synthesized cDNA solution to the PCR solution does not inhibit the PCR reaction.

The ReverTra Ace® qPCR RT Kit is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR. This kit contains the highly efficient reverse transcriptase "ReverTra Ace®" and a RT buffer optimized for the highly efficient synthesis of short-chain cDNAs suitable for real-time PCR. The protocol is simple, and the reaction can be completed in 15 min. ReverTra Ace® is a mutant-type M-MLV reverse transcriptase shows excellent efficiency.
ReverTra Ace® qPCR RT Master Mix (Code No. FSQ-201) is a premix version of ReverTra Ace® qPCR RT Kit. The master mix reagent (5x) contains the highly efficient reverse transcriptase "ReverTra Ace®" , primers and buffer optimized for highly efficient synthesis of short-chain cDNAs suitable for real-time PCR.

  • Code No. FSQ-101 200 reactions
  • Code No. FSQ-201 200 reactions (Premix Version)

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ReverTra Ace® qPCR RT Master Mix with gDNA Remover
High Efficient cDNA synthesis kit with gDNA remover

  • "Genomic DNA degradation step" and "cDNA synthesis step" are carried out continuously.
  • The optimized RT buffer and Primer Mix (oligo dT & random primers) enable the highly efficient reverse transcription.
  • Control experiment can be performed with 5x RT Master Mix II no RT-Control.

This kit includes master mix reagents for reverse transcription and for the removal of genomic DNA [DNase I treatment]. In many cases, total RNA prepared using spin-columns or acid guanidium-phenol- chloroform (AGPC) extraction methods contains small amount of genomic DNA. Any contaminating genomic DNA will be amplified along with cDNA, especially when primer pairs are designed within the same exon or from pseudogenes. Amplification from genomic DNA can result in qualitative and quantitative inaccuracies. The genomic DNA degradation and revers transcription steps can be achieved sequentially without purification or heat inactivation of DNase I.

  • Code No. FSQ-301 200 reactions

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SuperPrep® Cell Lysis & RT Kit for qPCR
Cultured cell lysis & cDNA Synthesis kit for real-time PCR

  • This kit enables to be obtained a high-quality cDNA from cultured cells.
  • A RNA purification step is not necessary.
  • Reduction of dispersion on high-throughput assay.

SuperPrep® Cell Lysis & RT Kit for qPCR (Code No. SCQ-101) consists of "Lysis Reagents" and "RT Reagents" for synthesis of cDNA templates for real-time PCR assays. "Lysis Reagents" can be used to prepare cell lysates containing RNAs that can be used as templates for reverse transcription.
"RT Reagents" can be used to carry out reverse transcription, and are optimized for efficient cDNA synthesis from crude lysates. The synthesized cDNA can be used as a template for two step real-time PCR. The cell lysate prepared by "Lysis Reagents" can be applied to one-step real-time PCR.

  • Code No. SCQ-101 100 reactions (This kit contain Lysis and RT Reagents.)
  • Code No. SCQ-201 100 reactions (This kit contain Lysis Reagent only.)

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SuperPrep® II Cell Lysis & RT Kit for qPCR
SuperPrep® II Cell Lysis Kit for qPCR
Cell lysis & cDNA Synthesis kit for real-time PCR (For cultured cells)

  • RNA purification is not necessary.
  • High-quality cDNA can be obtained from cell lysates.
  • Reduction of dispersion on high-throughput assay.
  • Various real-time PCR regents can be applied

SuperPrep® II Cell Lysis & RT Kit for qPCR (Code No. SCQ-401) consists of "Lysis Reagents" and "RT Reagents" for synthesis of cDNA templates for real-time PCR assays.
"Lysis Reagents" prepares cell lysates containing RNAs that can be used as templates for reverse transcription. "RT Reagents" contains reagents for reverse transcription, optimized for efficient cDNA synthesis from crude lysates. The synthesized cDNA can be applied to real-time PCR directly. This assay system is suitable for high-throughput assays. In the previous version, it was necessary to add Stop Solution after adding Lysis Solution. In SuperPrep® II, cell lysates with lysis solution can be used as templates for cDNA synthesis directly.

  • Code No. SCQ-401 100 reactions (This kit contain Lysis and RT Reagents.)
  • Code No. SCQ-501 100 reactions (This kit contain Lysis Reagent only.)

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