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PCR

Toyobo has various unique PCR enzymes, kits and PCR related products as shown below. The following tables show the characteristics of each product. Detailed information can be obtained by going to the linked sites.


PCR Enzyme & Kit

Enzyme Product Name Fidelity
(3'→5'
Exonuclease
activity)
Efficiency Velocity
(Extension
time)
Target
size
Crude
sample
Reverse
Transcriptase
activity
Hot start PCR Product
Ends
KOD DNA
polymerase
KOD -Plus- ++++
[80 folds]
++ +
(1 min/kb)
~12 kb + - blunt
KOD -Plus- Neo ++++
[80]
++ +++
(≤0.5 min/kb)
~24 kb + - blunt
KOD FX +++
[11]
+++ +
(1 min/kb)
~24 kb +++ - blunt
KOD FX Neo +++
[11]
+++ +++
(≤0.5 min/kb)
~40 kb ++++ - blunt
KOD Dash +
[3]
+++ +++
(≤0.5 min/kb)
~18 kb ++ - - mixed
(blunt &
3'-dA)
KOD exo(-) -
[1]
+++ +++
(≤0.5 min/kb)
~10 kb ++ - - 3'-dA
Tth DNA
polymerase
rTth DNA Polymerase -
[1]
+ +
(1 min/kb)
~2 kb +
(Mn2+)
- 3'-dA
RT-PCR Quick Master Mix -
[1]
+ +
(1 min/kb)
~2 kb + 3'-dA
Taq DNA
polymerase
rTaq DNA polymerase -
[1]
+ +
(1 min/kb)
~2 kb + - - 3'-dA
Quick Taq® HS DyeMix -
[1]
+ +
(1 min/kb)
~4 kb + - 3'-dA
Blend Taq® +
[3]
++ +
(1 min/kb)
~23 kb ++ - - mixed
(blunt &
3'-dA)
Blend Taq® -Plus- +
[3]
++ +
(1 min/kb)
~23 kb ++ -

++++: Best, +++: Excellent or Strong, ++: Good or Moderate, +: satisfactory, -: Not good or minus , : Applicable

[ ]: Accuracy of each PCR enzyme (reagent) when the accuracy of Taq DNA polymerase is set to 1.



PCR related product

Product Name Remarks
Anti-Taq high Anti-Taq DNA polymerase neutralization antibody for hot start
Deoxyribonucleotide triphosphate (dNTPs) Highly purified dNTPs for PCR
RNase Inhibitor Highly purified RNase inhibitor for reverse transcription
TArget Clone™ TA cloning of PCR products having overhanged dA at the 3'-ends. PCR products from Taq DNA polymerase, Blend Taq® [Code No. BTQ-101], Blend Taq® -Plus- [Code No. BTQ-201] or KOD Dash [Code No. LDP-101] are available
TArget Clone™ -Plus- TA cloning of blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201] or KOD FX [Code No. KFX-101].
10xA-attachment mix Attachment of dA at the 3' - ends of blunt end PCR products from KOD -Plus- and KOD FX ( For TA cloning )

PCR Enzyme & Kit

High fidelity PCR polymerase

KOD -Plus-

(click here)

  • The PCR error ratio of KOD -Plus- is approx. 80 times less than Taq DNA polymerase.
  • Hot Start technology results in highly efficient amplification.
  • KOD -Plus- enables the following amplifications (maximum): 21 kb from lambda phage DNA, 12 kb from human genomic DNA, and 7 kb from cDNA.

Code No. KOD-201 200 U 200 reactions


KOD -Plus- is based on DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1. KOD -Plus- exhibits excellent high PCR fidelity and efficiency. The enzyme solution of KOD -Plus- contains two types of anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR. KOD -Plus- generates blunt-end PCR products, due to 3'→5' exonuclease (proof-reading) activity.

High fidelity & efficient & fast PCR enzyme

KOD -Plus- Neo

(click here)

  • The PCR error ratio of KOD -Plus- Neo is about 80 times less than that of Taq DNA polymerase and is equal to the previous version (KOD -Plus-).
  • Requires only 30 sec/kb for the PCR extension step.
  • "Elongation enhancer" enables greater amplification efficiency and elongation capability (up to 24 kb from human genomic DNA) compared to conventional PCR.

Code No. KOD-401 200 U , 200 reactions


KOD -Plus- Neo is the improved version of the previous KOD -Plus- (Code No. KOD-201). This polymerase contains a unique "elongation enhancer" that suppresses the "plateau effect" produced by conventional PCR.
Therefore, this reagent exhibits greater amplification efficiency and elongation capability compared to the previous version of KOD -Plus-.
Moreover, this enzyme requires only 30 sec/kb for the PCR extension step. This facilitates the long range PCR.

High success-rate DNA polymerase

KOD FX

(click here)

  • Effective for the amplification of GC-rich targets and crude samples.
  • Hotstart technology using anti-KOD DNA polymerase antibodies enables highly efficient amplification.
  • KOD FX enables the following amplifications (Maximum): 40kb from lambda phage DNA, 24kb from human genomic DNA, and 13.5kb from cDNA.
  • The PCR error ratio of KOD FX is about 10 times less than that of Taq DNA polymerase.

Code No. KFX-101 200 U 200 reactions


KOD FX is based on DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1. KOD FX results in much greater PCR success based on efficiency and elongation capabilities than KOD -Plus- (Code No. KOD-201) or other Taq-based PCR enzymes. KOD FX enzyme solution contains two types of anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activities, thus allowing for Hot Start PCR. KOD FX generates blunt-end PCR products, due to 3'→5' exonuclease (proof-reading) activity.

High efficient & high success-rate PCR enzyme

KOD FX Neo

(click here)

  • KOD FX Neo is effective for amplification from crude samples (e.g. biological samples, foodstuffs, soil extract, etc). Various samples or lysates can be used directly as templates.
  • "Elongation enhancer" enables greater amplification efficiency and elongation capability (up to 40 kb from human genomic DNA) compared to conventional PCR. This enzyme is useful for amplifying difficult targets, such as high G/C, A/T, and/or long targets.
  • Various microorganisms (e.g. yeast, fungus, gram-positive bacteria) can be directly used as templates for PCR.
  • The PCR error ratio of KOD FX Neo is about 10 times less than that of Taq DNA polymerase and is equal to the previous version (KOD FX).

Code No. KFX-201 200 U , 200 reactions


KOD FX Neo is the improved version of the previous KOD FX (Code No. KFX-101). This product contains a unique "elongation enhancer" that suppresses the "plateau effect", enabling greater elongation rates and capabilities.
The KOD FX Neo enzyme solution contains two types of anti-KOD DNA polymerase antibodies that inhibit the polymerase and 3'→5' exonuclease activities, thus allowing for Hot Start PCR. KOD FX Neo is a highly efficient enzyme that is tolerant of PCR inhibitors in crude samples.

High Speed & Efficient DNA polymerase

KOD Dash

(click here)

  • Shows greater elongation velocity than Taq DNA polymerase (2 folds) and Pfu DNA polymerase (6 folds) due to the property of KOD DNA polymerase.
  • The PCR error ratio of this enzyme mixture is approximately 3 to 4 times less than that of Taq DNA polymerase.
  • Effective for the amplification of various targets from a small template amount.

Code No. LDP-101 250 U 200 reactions


KOD Dash is a highly efficient DNA polymerase mixture developed based on the Barns' method. This method uses a DNA polymerase which lacked a 3'→5' exonuclease (proofreading) activity and a small amount of an archaeal DNA polymerase with proofreading activity. In the reagent, the 3'→5' exonuclease activity-minus mutant <KOD EXO(-)> of KOD DNA polymerase and KOD DNA polymerase are used. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of a polymerase reaction, PCR with this mixed enzyme solution enables highly efficient amplification. KOD Dash generates dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA cloning method.

High Speed DNA polymerase

KOD exo (-)

(click here)

  • Shows greater elongation velocity than Taq DNA polymerase (2 folds) and Pfu DNA polymerase (6 folds) due to the property of KOD DNA polymerase.
  • The PCR error ratio of this enzyme mixture is approximately equal to that of Taq DNA polymerase.
  • This enzyme is effective for the amplification of various targets from a small template amount.

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KOD exo (-) is a 3'→5' exonuclease minus mutant developed based on KOD DNA polymerase from a hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1. KOD exo (-) shows excellent elongation velocity.

DNA polymerase having reverse
transcriptase activity

rTth DNA polymerase

(click here)

  • Exhibits reverse transcriptase activity in addition to DNA polymerase activity in the presence of Mn2+ ions.

Code No. TTH-301 250 U 100-200 reactions


rTth DNA polymerase is a recombinant DNA polymerase derived from the thermophilic bacteria Thermus thermophilus (Tth) HB8. This polymerase exhibits reverse transcriptase activity in addition to DNA polymerase activity in the presence of Mn2+ ions.Therefore, this enzyme enables “one-step RT-PCR” including reverse transcription and PCR steps.

One-step RT-PCR Master Mix

RT-PCR Quick Master Mix

(click here)

  • Suitable for high-throughput RT-PCR.
  • This kit has the advantage of amplifying targets with complex conformations and GC-rich content.
  • Hot-start technology using anti-Tth DNA polymerase antibodies enables highly efficient and specific amplification.

Code No. PCR-311 50 reactions


RT-PCR Quick Master Mix provides a 2 × Master Mix for RT-PCR using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in addition to DNA polymerase activity in the presence of Mn2+ ions. Therefore, this system enables “one-step RT-PCR” including reverse transcription and PCR steps. This kit is suitable for high-throughput RT-PCR, and decreases contamination risks.

Standard PCR Enzyme

rTaq DNA polymerase

(click here)


Code No. TAP-201 250 U 100-200 reactions


rTaq DNA polymerase is a recombinant DNA polymerase derived from the thermophilic bacteria Thermus aquaticus (Taq) YT-1.

Taq DNA polymerase (Dye-premix & Hot-start version)

Quick Taq® HS DyeMix

(click here)

  • The hot start technology enables efficient greater PCR performance than conventional Taq DNA polymerase.
  • The master mix is stable for at least three months at 4ºC. No decrease in reaction efficiency is observed following 30 freeze-thaw cycles.

Code No DTM-101 100 reactions


Quick Taq® HS DyeMix is a Taq-based 2 × master mix PCR reagent that contains an electrophoresis dye (BPB; bromophenol blue) and anti-Taq antibodies for hot start PCR. This reagent contains all components for PCR except primers and template DNA. This reagent shows specific and efficient amplification. The amplified products can be directly loaded in the wells of an agarose or acrylamide gel.

High Efficient Taq DNA polymerase

Blend Taq® / Blend Taq® -Plus-

(click here)

  • The elongation ability of this enzyme is much greater than that of the normal Taq DNA polymerase.
  • Hot Start technology using anti-Taq DNA polymerase antibodies results in highly efficient amplification. <Blend Taq® (Code No. BTQ-101) does not use hot start>
  • The PCR error ratio of this enzyme is approximately 3-4 times less than that of Taq DNA polymerase.

Code No. BTQ-101 250 U 200 reactions <Blend Taq®>

Code No. BTQ-201 250 U 200 reactions <Blend Taq® -Plus->


Blend Taq® and Blend Taq® -Plus- are highly efficient Taq-based DNA polymerases developed based on the Barns' method. This method uses a DNA polymerase which lacked 3'→5' exonuclease (proofreading) activity (e.g., Taq DNA polymerase) and a small amount of an archaeal DNA polymerase with proofreading activity. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of the polymerase reaction, PCR with a ‘mixed' enzyme solution enables highly efficient amplification. The enzyme solution of Blend Taq® -Plus- contains anti-Taq DNA polymerase antibodies that inhibit polymerase activity, allowing for Hot Start PCR. Blend Taq® and Blend Taq® -Plus- generate dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA-cloning method.

PCR related product

Anti-Taq DNA polymerase antibody

Anti-Taq high

(click here)

Code No. TCP-101 100μl


This product is a highly purified neutralization monoclonal antibody to Taq DNA polymerase. The product can be applied for hot start PCR using Taq DNA polymerase by mixing with the Taq DNA polymerase solution.

dNTPs Mixture(2mM)

dNTPs Mixture(10mM)

(click here)

  • 2 mM and 10 mM solutions are available
  • Suitable for PCR and reverse transcriptation

Code No. NTP-201 2 µmoles/1 ml (2 mM)

Code No. NTP-301 2 µmoles/0.2 ml (10 mM)


dNTPs Mixture is an equal moler solution mixture of ultrapure dATP, dCTP, dGTP and dTTP.

RNase inhibitor

(click here)

Code No. SIN-201 2,500 U


This product is a recombinant type human ribonuclease inhibitor (RNase inhibitor; 51kDa) that is produced by E. coli. RNase inhibitor forms a complex with ribonuclease A (RNase A) and inhibits the activity of RNase A. This inhibitor can be applied to reverse transcriptase reactions.

High efficient TA cloning kit

TArget Clone™

(click here)

  • Ligation step can be completed in 5 min with high efficiency.
  • PCR products can be used without purification.

Code No. TAK-101 10 reactions


TArget Clone™ [Code No. TAK-101] can be applied to the TA cloning of PCR products amplified using Taq DNA polymerase, Blend Taq® [Code No. BTQ-101], Blend Taq® -Plus- [Code No. BTQ-201] or KOD Dash [Code No. LDP-101]. Almost all PCR products having dA overhanging at the 3'end are available.

High efficient TA cloning kit for KOD products

TArget Clone™ -Plus-

(click here)

  • Can be applied to the TA cloning of blunt-end PCR products of KOD DNA polymerase.
  • Ligation step can be completed in 5 min with high efficiency.
  • PCR products can be used without purification.

Code No. TAK-201 10 reactions


TArget Clone™ -Plus- [Code No. TAK-201] can be applied to the TA cloning of blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201, KOD-401] or KOD FX [Code No. KFX-101, KFX-201]. TArget Clone™ -Plus- contains 10xA-attachment mix. This reagent is a mixture of anti-KOD DNA polymerase antibody specific to KOD 3'→5' exonuclease activity (proof-reading activity) and Taq DNA polymerase, which exhibits terminal transferase activity. The 10 x A-attachment mix allows for blunt end PCR products to acquire overhanging dA at the 3'-ends

dA attachment reagent

10xA-attachment mix

(click here)

  • The attachment reaction is completed in 10 minutes at 60ºC.
  • The cloning efficiency of dA attached PCR products is as great as that of PCR products of Taq DNA polymerase.

Code No. TAK-301 25 reactions


10 x A-attachment mix is a reagent consists of the anti-KOD DNA polymerase antibody for the 3'→5' exonuclease activity (proof-reading activity) of the enzyme, and Taq DNA polymerase exhibits the terminal transferase activity. The PCR products from KOD -Plus- [Code No. KOD-201] and KOD FX [Code No. KFX-101] have blunt ends due to the 3'→5' exonuclease activity of KOD DNA polymerase. 10 x A-attachment mix allows the PCR products to acquire overhanging dA at the 3'-ends. The products having 3'-dA overhang can be directly cloned into arbitrary T-vectors using ligation reagents such as Ligation high Ver.2 [Code No. LGK-201].