Blend Taq® and Blend Taq® -Plus- are highly efficient Taq-based DNA polymerases developed based on the Barns' method(1). This method uses a DNA polymerase which lacked 3'→5' exonuclease (proofreading) activity (e.g., Taq DNA polymerase) and a small amount of an archaeal DNA polymerase with proofreading activity. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of the polymerase reaction, PCR with a 'mixed' enzyme solution enables highly efficient amplification.
The enzyme solution of Blend Taq® -Plus- contains anti-Taq DNA polymerase antibodies that inhibit polymerase activity, allowing for Hot Start PCR.
Blend Taq® and Blend Taq® -Plus- generate dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA-cloning method.