Real-time PCR Kits

Toyobo has various real-time PCR reagents showing below. The following table shows the characteristics of each product. The detailed information can be referred in the linked sites.

Enzyme Product Name Assay
type
One-
step
RT-
PCR
Hot
start
(Anti-
body)
Pas-
sive
ref-
er-
ence
Glass
capil-
lary
Spec-
ificity
Effi-
cien-
cy
Long
target
ampli-
fica-
tion
GC-ri-c
h tar-
gets
Amplifi-
cation
from
crude
samples
Taq DNA polymerase Realtime PCR Master Mix Probe   +++ ++ + + +
SYBR® Green Realtime PCR Master Mix (-Plus-) SYBR®   + ++ + + +
TUNDERBIRD® Probe qPCR Mix Probe   +++ ++ + + +
TUNDERBIRD® SYBR® qPCR Mix SYBR®   ++ ++ + + +
Tth DNA polymerase [One Step] RNA-direct™ Realtime PCR Master Mix Probe +++ ++ + ++ ++
RNA-direct™ SYBR® Green Realtime PCR Master Mix SYBR® ++ ++ + ++ ++
THUNDERBIRD™ Probe One-step qRT-PCR Kit Prode +++ +++ + ++ ++
KOD exo(-) DNA polymerase KOD SYBR® qPCR Mix SYBR®   ++ ++ +++
(<2 kb)
+++ +++
GenNext™ NGS Library Quantification Kit SYBR®     ++ ++ +++
(<2 kb)
+++ +++

+++: BEST, ++: Excellent, +: Good, ✓: Applicable

Realtime PCR Master Mix Efficient qPCR Master Mix for Probe Assay

  • Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible probe assay.
  • Can be used in glass capillary systems (e.g. LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in a passive reference system (e.g., ABI PRISM®7700, Applied Biosystems, Inc.).

This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer and probe. This reagent is applicable in TaqMan® assays or hybridization probe assays, in combination with each probe.

  • Code No. QPK-101 1 mL x 5 200 reactions

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SYBR® Green Realtime PCR Master Mix Efficient qPCR Master Mix for Intercalation Assay

  • Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible intercalation assay.
  • Can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in a passive reference system (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.).

This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for intercalation assay with SYBR® Green I.

  • Code No. QPK-201 1 mL x 5 200 reactions

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SYBR® Green Realtime PCR Master Mix -Plus- Improved qPCR Master Mix for Intercalation Assay

  • Hot Start technology with anti-Taq DNA polymerase antibodies and additives enable excellent specificity and reproducible intercalation assay.
  • Can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • This reagent can be used in a passive reference system (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.).

This product is an improved version of a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for highly specific intercalation assay with SYBR® Green I. This reagent is effective for reducing non-specific amplifications or primer dimers.

  • Code No. QPK-212 1 mL x 5 200 reactions

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THUNDERBIRD® qPCR Mix High efficient real-time PCR Master Mix

  • The specificity for the detection of low-copy targets is improved.
  • The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending).
  • High specificity and effective amplification enable the detection of a broad dynamic range.
  • The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.

THUNDERBIRD® Probe and SYBR® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using TaqMan® probes and SYBR® Green I. The master mix contains all required components, except for ROX reference dye, probe and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments.
These products are improved versions of Realtime PCR Master Mix (Code No. QPK-101) and SYBR® Green Realtime PCR Master Mix (Code No. QPK-201) .

  • Code No. QPS-101 200 reactions (TaqMan® probe version)
  • Code No. QPS-201 200 reactions (SYBR® Green version)

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RNA-direct™ Realtime PCR Master Mix One-step qPCR Master Mix for Probe Assay

  • Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
  • Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in systems using passive reference (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems.
  • Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible probe assay.

This product is a 2 × Master Mix for "1-step real-time PCR" using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for "1-step real-time PCR", including reverse transcription and PCR steps. This reagent is applicable for TaqMan® assay or hybridization probe assay, in combination with each probe.

  • Code No. QRT-101 0.5 mL x 5 100 reactions

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RNA-direct™ SYBR® Green Realtime PCR Master Mix
One-step qPCR Master Mix for Intercalation Assay

  • Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
  • Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in systems using passive reference (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems.
  • Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible intercalation assay.

This product is a 2 × Master Mix for "1-step real-time PCR" using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for "1-step real-time PCR", including reverse transcription and PCR steps. This reagent can be applied to an intercalation assay with SYBR® Green I.

  • Code No. QRT-201 0.5 mL x 5 100 reactions

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THUNDERBIRD Probe One-step qRT-PCR Kit High Efficient One-step qRT-PCR Kit

  • Rapid and highly sensitive
  • Tolerant of target sequence diversity
  • Tolerant of PCR inhibitors
  • Utilization of dUTP
  • Multiplex detection

THUNDERBIRD Probe One-step qRT-PCR Kit is a one-step real-time reverse-transcription polymerase chain reaction (RT-PCR) kit using the highly efficient reverse transcriptase "ReverTra Ace®" and Tth DNA polymerase as a PCR enzyme. This product can be used mainly in TaqMan® probe assays. The one-step system is suitable for high-throughput analysis because of its simple reaction setup. In addition, this system can reduce the risk of cross-contamination.

  • Code No. QRZ-101 100 reactions

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KOD SYBR® qPCR Mix High efficient real-time PCR Master Mix with KOD

  • Quantitative analysis can be achieved using long targets, up to 2kb.
  • Because of using KOD DNA polymerase, the product is tolerant of PCR inhibitors in crude samples.
  • Enable the effective amplification from GC-rich targets.

KOD SYBR® qPCR Mix is a highly efficient 2× Master Mix for real-time PCR using SYBR® Green I and based on the 3’-5’ exonuclease deficient KOD DNA Polymerase [KOD exo(-)]. The master mix contains all the required components, except the ROX reference dye and primers (50x ROX reference dye is supplied separately with this kit). The master mix aids reaction setup, and improves the reproducibility of experiments.

  • Code No. QKD-201 200 reactions

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GenNext NGS Library Quantification Kit Library quantification kit for illumina

  • Accurate quantification
    KOD SYBR® qPCR Mix can efficiently amplify GC- and AT-rich fragments of different lengths without bias.
  • Broad dynamic range
    The kit has a broad dynamic range from 20 pM (Standard DNA 1) to 0.0002 pM (Standard DNA 6).
  • Convenient
    The kit contains all reagents (KOD SYBR® qPCR Mix, 5x Primer Mix, Standard DNA, and 50x Dilution buffer) needed for the qPCR-based quantification of an NGS library.

GenNext™ NGS Library Quantification Kit is for the SYBR® Green I qPCR-based library quantification of Illumina next-generation sequences. The kit allows the specific and accurate quantification of libraries bearing P5 and P7 adaptors which can be applied to flow cell amplification. It uses the highly efficient qPCR master mix KOD SYBR® qPCR Mix.

  • Code No. NLQ-101 500 reactions

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