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Real-time PCR Kits

Toyobo has various real-time PCR reagents showing below. The following table shows the characteristics of each product. The detailed information can be referred in the linked sites.

Enzyme Product Name Assay type One-step RT-PCR Hot start (Antibody) Passive reference Glass capillary Specificity Efficiency Long target amplification GC-rich targets Amplification from crude samples
Taq DNA polymerase Realtime PCR Master Mix Probe   check check check +++ ++ + + +
SYBR® Green Realtime PCR Master Mix (-Plus-) SYBR®   check check check + ++ + + +
TUNDERBIRD® Probe qPCR Mix Probe   check check check +++ ++ + + +
TUNDERBIRD® SYBR® qPCR Mix SYBR®   check check check ++ ++ + + +
Tth DNA polymerase [One Step] RNA-direct™ Realtime PCR Master Mix Probe check check check check +++ ++ + ++ ++
RNA-direct™ SYBR® Green Realtime PCR Master Mix SYBR® check check check check ++ ++ + ++ ++
KOD exo(-) DNA polymerase KOD SYBR® qPCR Mix SYBR®   check check check ++ ++ +++
(<2 kb)
+++ +++

+++: BEST, ++: Excellent, +: Good, : Applicable



Efficient qPCR Master Mix for Probe Assay

Realtime PCR Master Mix

(click here)

  • Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible probe assay.
  • Can be used in glass capillary systems (e.g. LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in a passive reference system (e.g., ABI PRISM®7700, Applied Biosystems, Inc.).

Code No. QPK-101T 1 mL x 1     40 reactions

Code No. QPK-101   1 mL x 5   200 reactions


This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer and probe. This reagent is applicable in TaqMan® assays or hybridization probe assays, in combination with each probe.

Efficient qPCR Master Mix for Intercalation Assay

SYBR® Green
Realtime PCR Master Mix

(click here)

  • Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible intercalation assay.
  • Can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in a passive reference system (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.).

Code No. QPK-201T 1 mL x 1     40 reactions

Code No. QPK-201   1 mL x 5   200 reactions


This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for intercalation assay with SYBR® Green I.

Improved qPCR Master Mix for Intercalation Assay

SYBR® Green Realtime PCR Master Mix -Plus-

(click here)

  • Hot Start technology with anti-Taq DNA polymerase antibodies and additives enable excellent specificity and reproducible intercalation assay.
  • Can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • This reagent can be used in a passive reference system (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.).

Code No. QPK-212T 1 mL x 1     40 reactions

Code No. QPK-212   1 mL x 5   200 reactions


This product is an improved version of a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for highly specific intercalation assay with SYBR® Green I. This reagent is effective for reducing non-specific amplifications or primer dimers.

High efficient real-time PCR Master Mix

THUNDERBIRD® qPCR Mix

(click here)

  • The specificity for the detection of low-copy targets is improved.
  • The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending).
  • High specificity and effective amplification enable the detection of a broad dynamic range.
  • The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.

Code No. QPS-101 200 reactions (TaqMan® probe version)

Code No. QPS-201 200 reactions (SYBR® Green version)


THUNDERBIRD® Probe and SYBR® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using TaqMan® probes and SYBR® Green I. The master mix contains all required components, except for ROX reference dye, probe and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments.
These products are improved versions of Realtime PCR Master Mix (Code No. QPK-101) and SYBR® Green Realtime PCR Master Mix (Code No. QPK-201) .

One-step qPCR Master Mix for Probe Assay

RNA-direct
Realtime PCR Master Mix

(click here)

  • Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
  • Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in systems using passive reference (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems.
  • Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible probe assay.

Code No. QRT-101T 0.5 mL x 2     40 reactions

Code No. QRT-101   0.5 mL x 5   100 reactions


This product is a 2 × Master Mix for “1-step real-time PCR” using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for “1-step real-time PCR”, including reverse transcription and PCR steps. This reagent is applicable for TaqMan® assay or hybridization probe assay, in combination with each probe.

One-step qPCR Master Mix for Intercalation Assay

RNA-direct™ SYBR® Green Realtime PCR Master Mix

(click here)

  • Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
  • Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in systems using passive reference (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems.
  • Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible intercalation assay.

Code No. QRT-201T 0.5 mL x 2     40 reactions

Code No. QRT-201   0.5 mL x 5   100 reactions


This product is a 2 × Master Mix for “1-step real-time PCR” using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for “1-step real-time PCR”, including reverse transcription and PCR steps. This reagent can be applied to an intercalation assay with SYBR® Green I.

High efficient real-time PCR Master Mix with KOD

KOD SYBR® qPCR Mix

(click here)

  • Quantitative analysis can be achieved using long targets, up to 2kb.
  • Because of using KOD DNA polymerase, the product is tolerant of PCR inhibitors in crude samples.
  • Enable the effective amplification from GC-rich targets.

Code No. QKD-201 200 reactions


KOD SYBR® qPCR Mix is a highly efficient 2× Master Mix for real-time PCR using SYBR® Green I and based on the 3’-5’ exonuclease deficient KOD DNA Polymerase [KOD exo(-)]. The master mix contains all the required components, except the ROX reference dye and primers (50x ROX reference dye is supplied separately with this kit). The master mix aids reaction setup, and improves the reproducibility of experiments.