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Real-time PCR Master Mix

Toyobo has various real-time PCR reagents showing below. The following table shows the characteristics of each product. The detailed information can be referred in the linked sites.

Enzyme Product Name SYBR Green Assay Probe Assay Hot start Passive reverence Specificity Efficiency
Taq DNA polymerase Realtime PCR Master Mix   check check check ++ ++
SYBR Green® Realtime PCR Master Mix check   check check + ++
SYBR Green® Realtime PCR Master Mix -Plus- check   check check ++ +(+)
ReverTra Ace® ReverTra Ace qPCR RT Kit High efficient reverse transcription kit for real-time PCR
Tth DNA polymerase RNA-direct™
Realtime PCR Master Mix
  check check check ++ ++
RNA-direct™
SYBR Green® Realtime PCR Master Mix
check   check check + ++

++: Excellent, +: Good, : Applicable



Efficient qPCR Master Mix for Probe Assay

Realtime PCR Master Mix

(click here)

  • Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible probe assay.
  • Can be used in glass capillary systems (e.g. LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in a passive reference system (e.g., ABI PRISM®7700, Applied Biosystems, Inc.).

Code No. QPK-101T 1 mL x 1     40 reactions

Code No. QPK-101   1 mL x 5   200 reactions


This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer and probe. This reagent is applicable in TaqMan® assays or hybridization probe assays, in combination with each probe.

Efficient qPCR Master Mix for Intercalation Assay

SYBR® Green
Realtime PCR Master Mix

(click here)

  • Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible intercalation assay.
  • Can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in a passive reference system (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.).

Code No. QPK-201T 1 mL x 1     40 reactions

Code No. QPK-201   1 mL x 5   200 reactions


This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for intercalation assay with SYBR® Green I.

Improved qPCR Master Mix for Intercalation Assay

SYBR® Green Realtime PCR Master Mix -Plus-

(click here)

  • Hot Start technology with anti-Taq DNA polymerase antibodies and additives enable excellent specificity and reproducible intercalation assay.
  • Can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • This reagent can be used in a passive reference system (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.).

Code No. QPK-212T 1 mL x 1     40 reactions

Code No. QPK-212   1 mL x 5   200 reactions


This product is an improved version of a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for highly specific intercalation assay with SYBR® Green I. This reagent is effective for reducing non-specific amplifications or primer dimers.

High Efficient Reverse Transcription Kit
for Real-time PCR

ReverTra Ace®
qPCR RT Kit

(click here)

  • The optimized RT buffer and Primer Mix enable the highly efficient reverse transcription from RNA to cDNA without additional requirements.
  • Can be completed in 15 min. The protocol does not contain an additional RNase H treatment step to remove residual RNA after reverse transcription.
  • Since the RT buffer is optimized for real-time PCR, the addition of 20% (v/v) of the synthesized cDNA solution to the PCR solution does not inhibit the PCR reaction. Therefore, this kit is suitable for the detection of low-expressing mRNAs.

Code No. FSQ-101 200 reactions


The ReverTra Ace® qPCR RT Kit is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR. This kit contains the highly efficient reverse transcriptase “ReverTra Ace®” and a RT buffer optimized for the highly efficient synthesis of short-chain cDNAs suitable for real-time PCR. The protocol is simple, and the reaction can be completed in 15 min. ReverTra Ace® is a mutant-type M-MLV reverse transcriptase shows excellent efficiency. This kit is suitable for preparation of cDNA for Realtime PCR Master Mix series reagents (Code No. QPK-101, 201, 211).

One-step qPCR Master Mix for Probe Assay

RNA-direct
Realtime PCR Master Mix

(click here)

  • Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
  • Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in systems using passive reference (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems.
  • Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible probe assay.

Code No. QRT-101T 0.5 mL x 2     40 reactions

Code No. QRT-101   0.5 mL x 5   100 reactions


This product is a 2 × Master Mix for “1-step real-time PCR” using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for “1-step real-time PCR”, including reverse transcription and PCR steps. This reagent is applicable for TaqMan® assay or hybridization probe assay, in combination with each probe.

One-step qPCR Master Mix for Intercalation Assay

RNA-direct™ SYBR® Green Realtime PCR Master Mix

(click here)

  • Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
  • Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in systems using passive reference (e.g., ABI PRISM® 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems.
  • Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible intercalation assay.

Code No. QRT-201T 0.5 mL x 2     40 reactions

Code No. QRT-201   0.5 mL x 5   100 reactions


This product is a 2 × Master Mix for “1-step real-time PCR” using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for “1-step real-time PCR”, including reverse transcription and PCR steps. This reagent can be applied to an intercalation assay with SYBR® Green I.