MAGNETIC BEADS TECHNOLOGY
Silica beads can be used to extract various kinds of nucleic acids based on binding properties of DNA to a silica surface in the presence of chaotropic agents (1), (2).
The "magnetic" silica beads facilitate high-throughput extraction of nucleic acids using magnets and can be easily automated.
Fig. 1. Magnetic silica beads
Fig. 2. Magnetic separation of magnetic silica beads
Fig. 3. Principle of purification
The selectivity of extracted nucleic acids can be changed by optimization of the binding and washing solutions. MagExtractor -Genome- (Code No. NPK-101) extracts genomic DNA from various specimens (e.g. whole blood, cultured cells or animal tissues etc.). MagExtractor -RNA- (Code No. NPK-201F) extracts total RNA from various specimens (e.g. cultured cells or animal tissues).
MagExtractor -Plasmid- (Code No. NPK-301) extracts plasmids from E. coli cells, MagExtractor -Viral RNA- (Code No. NPK-401F) is a kit for extracting viral RNA from serum or plasma specimens. MagExtractor -Plant Genome- (Code No. NPK-501) is a kit for extracting genomic DNA from various plant specimens (e.g., leaf, cultured cells, etc.). MagExtractor-PCR & Gel Clean up- (Code No. NPK-601) extracts DNA fragments from a PCR solution, enzyme solution, or agarose gel slices.
Fig. 4. Magnetic stand: Magical Trapper
Magical Trapper (Code No. MGS-101) is a convenient magnetic stand which can be applied to ten 1.5-2.0 ml-microtubes and one 15 ml centrifuge tube..
- B. Vogelstein and D. Gillespie, Proc. Natl. Acad. Sci. USA. 76: 615-619 (1979)
- R. Boom, C. J. A. Sol, M. M. M.Salimans, C. L. Wertheim-van Dillen, P. M. E. Dillen and J. van der Noordaa,
J. Clin. Microbiol., 28: 495-503 (1990)