MAGNETIC BEADS TECHNOLOGY

Silica beads can be used to extract various kinds of nucleic acids based on binding properties of DNA to a silica surface in the presence of chaotropic agents (1), (2).
The "magnetic" silica beads facilitate high-throughput extraction of nucleic acids using magnets and can be easily automated.

  • Fig. 1. Magnetic silica beads

  • Fig. 2. Magnetic separation of magnetic silica beads

  • Fig. 3. Principle of purification

The selectivity of extracted nucleic acids can be changed by optimization of the binding and washing solutions. MagExtractor -Genome- (Code No. NPK-101) extracts genomic DNA from various specimens (e.g. whole blood, cultured cells or animal tissues etc.). MagExtractor -RNA- (Code No. NPK-201F) extracts total RNA from various specimens (e.g. cultured cells or animal tissues).
MagExtractor -Plasmid- (Code No. NPK-301) extracts plasmids from E. coli cells, MagExtractor -Viral RNA- (Code No. NPK-401F) is a kit for extracting viral RNA from serum or plasma specimens. MagExtractor -Plant Genome- (Code No. NPK-501) is a kit for extracting genomic DNA from various plant specimens (e.g., leaf, cultured cells, etc.). MagExtractor-PCR & Gel Clean up- (Code No. NPK-601) extracts DNA fragments from a PCR solution, enzyme solution, or agarose gel slices.

Fig. 4. Magnetic stand: Magical Trapper

Magical Trapper (Code No. MGS-101) is a convenient magnetic stand which can be applied to ten 1.5-2.0 ml-microtubes and one 15 ml centrifuge tube..

REFERENCES

  • B. Vogelstein and D. Gillespie, Proc. Natl. Acad. Sci. USA. 76: 615-619 (1979)
  • R. Boom, C. J. A. Sol, M. M. M.Salimans, C. L. Wertheim-van Dillen, P. M. E. Dillen and J. van der Noordaa,
    J. Clin. Microbiol., 28: 495-503 (1990)