TOYOBO Ideas & Chemistry Biotechnology Operating
Department

PREPARATION and SPECIFICATION

Appearance White amorphous powder
Grade GradeⅢ
Contaminants Alkaline phosphatase Not detected＊1
Peroxidase Not detected＊2
Nentral Proteinase Not detected＊3

PROPERTIES

Stability Stable at 25℃ for at least one year （Fig.3）
Average molecular weight approximately 30,000 (hydrolysate)

APPLICATIONS

Neo Protein Saver is useful for stabilizing enzymes and conjugated antibodies. （Fig.1, 2）

(＊1 ≤9×10-4 U/mg, ＊2 ≤4×10-2 U/mg, ＊3 ≤5×10-1 U/mg)

Examples

  • Fig.1. NPS-BSA Comparison:Stabilizing Efficiency 1 (Liquid form Enzyme)

    Fig.1. NPS-BSA Comparison: Stabilizing Efficiency 1 (Liquid form Enzyme)

    Remaining activity for Peroxidase(5U/ml PEO-302) in 50mM PIPES-NaOH solution after 60days at 25℃ or 14days at 50℃

  • Fig.2. NPS-BSA Comparison: Stabilizing Efficiency 2 (Powder form Enzyme)

    Fig.2. NPS-BSA Comparison: Stabilizing Efficiency 2 (Powder form Enzyme)

    Remaining activity for Choresterol oxidase(COO-321 containing K-phosphate buffer) after 14days at 50℃
    ＊Equivalent amount of NPS-301 or BSA are added on enzyme protein

  • Fig.3. Stability (Powder form)

    Fig.3. Stability (Powder form)

    Stability of NPS after 6 or 12 months, measured by stabilizing efficiency for Peroxidase at the same condition with Fig.1. after 14 days at 50℃ NPS were kept under dry conditions.