TOYOBO Ideas & Chemistry Biotechnology Operating
Department

1. Safety guide for handling enzymes

Please read the following safety tips/guidelines before handling the enzymes.

APPLICATIONS

TOYOBO enzymes are obtained from various microorganisms, animals and plants. They are mainly used diagnostically, and are NOT used to treat humans or animals.

POSSIBLE RISKS

POSSIBLE RISK

Eye/skin irritation, coughing, or other allergic reactions may occur with direct contact, or inhalation or ingestion of the enzymes, which can lead to more severe symptoms such as asthma.

FIRST AID

FIRST AID

Eye/skin contact: Wash off with copious amounts of water.
Inhalation: Go outside into the fresh air and breathe deeply. Also gargle.
Ingestion: Swallow copious amounts of water to facilitate immediate vomiting.

* In any of the above events, promptly consult a physician afterwards.

PERSONAL PROTECTION

PERSONAL PROTECTION

Wear gloves, goggles, mask, and long-sleeved work clothes.

Note: The above safety guide is for the situation in which trained laboratory personnel are handling enzymes for diagnostic application, complying with appropriate safety procedures, for which the users are responsible. Should you use the enzymes for another application, be sure to provide safety measures corresponding to that application.

The sole purpose of this document is to offer information that is currently available, and a guideline. It does not constitute supplier's liability or a warranty.

For more information on enzyme composition, properties and characteristics, and hazard identification, the Material Safety Data Sheet is available upon request.

2. To keep the enzymes active

Enzymes may lose their activity because of humidity, increase in temperature, or other unsuitable conditions during storage or upon usage.
Refer to the following points to maintain their activity.

PERSONAL PROTECTION

The optimal storage temperature may vary between enzyme forms. Keep lyophilized enzymes frozen at -20ºC, and liquid enzymes refrigerated at 4ºC. Containers should remain unopened, in a dark, low-humidity environment. Some enzymes decompose when exposed to light.

AT THE TIME OF USE (LYOPHILIZED ENZYMES)

After removing the enzyme container from the freezer, do not open it immediately, but leave it unopened at room temperature for at least 3 hours, giving the enzyme time to adjust to this temperature; it may absorb moisture and lose activity in the event of rapid temperature increase. On opening the container, take the following steps to prevent the enzyme popping out of the container in a fluffy shape, because the change in temperature may slightly increase the internal pressure.
  • (1)Remove the sealing tape from the cap.
  • (2)Slightly loosen the cap, and leave it undisturbed for 1-2 minutes, allowing the pressure to adjust.
  • (3)Slowly remove the cap (and inner cap, if present).