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Raw Materials
Toyobo has various unique PCR enzymes and PCR related products as shown in the table below. Detailed information can be obtained by going to the linked sites.
| Enzyme | Product Name |
|---|---|
| Polymerases | |
| Antibodies | |
| Reverse Transcriptase | |
| Other Enzymes | |
| Buffers | 2x Buffer for rTth/TTx (DNA) 5x Buffer for rTth/TTx (DNA/RNA) |
| Glycerol Free Formulations | TTx DNA polymerase Anti-Taq DNA polymerase antibody Uracil DNA glycosylase |
| Monoclonal Antibodies | |
| dNTPs | |
| Other |
Mg solution Mn solution 50x ROX reference dye |
Bulk Products
We also offer some research reagents as bulk products.
Please click here for a list of research reagents.
rTaq DNA polymerase
Taq DNA polymerase is the most widely used thermostable DNA polymerase derived from the thermophilic bacteria Thermus aquaticus (Taq) YT-1. This enzyme possesses 5’→3’ polymerase activity and double-strand specific 5’→ 3’ exonuclease activity.
rTth DNA polymerase / Hot Start rTth DNA polymerase
Tth DNA polymerase is a thermostable DNA polymerase derived from the thermophilic bacteria Thermus thermophilus (Tth) HB8.
This enzyme has reverse transcriptase activity in addition to 5’→3’ polymerase activity and double strand specific 5’→ 3’ exonuclease activity in the presence of Mn2+ ions. Therefore, this enzyme enables "one-step RT-PCR", combining both the reverse transcription and PCR steps.
TTx DNA polymerase / Hot Start TTx DNA polymerase
TTx DNA polymerase is relatively tolerant of typical PCR inhibitors. TTx is useful to amplify DNA from crude samples. This enzyme exhibit reverse transcriptase activity in the presence of Mn2+ ions and can be used for one-step RT-PCR, combining the reverse transcription and PCR steps. These polymerases also exhibit double strand specific 5’ →3’ exonuclease activity and can be used in the TaqMan probe assay.
KOD DNA polymerase / Hot Start KOD DNA polymerase
KOD DNA polymerase is a thermostable DNA polymerase derived from the the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1. This enzyme possesses 5’→3’ polymerase activity and double-strand specific 3'→5' exonuclease (proof-reading) activity.
KOD exo(-) DNA polymerase
KOD DNA polymerase exhibits an excellent elongation capability (approx. 130 bases/sec) and strong 3’→5’ exonuclease activity (proofreading activity). However, its sensitivity and efficiency are subject to interference from the enzyme or template concentration during PCR, due to its strong proofreading activity. KOD exo(-) is a 3’→5’ exonuclease minus mutant developed based on KOD DNA polymerase from a hyperthermophilic Archaeon Thermococcus kodakaraensis.
Anti-Taq DNA polymerase antibody
Anti-Taq high is a highly purified neutralization monoclonal antibody for Taq DNA polymerase, rTth DNA polymerase, and TTx DNA polymerase. This product provides an antibody-mediated hot start PCR to enhance the specificity and sensitivity of PCR. This antibody inhibits polymerase activity before the onset of thermal cycling, preventing primer dimer formation and non-specific amplification. At the first denaturation step of the thermal cycling, the antibody is quickly inactivated and PCR proceeds. The antibody-mediated hot start method is more convenient to use than other hot start methods.
Anti-KOD DNA polymerase antibody
Anti-KOD DNA polymerase antibody is a highly purified neutralization monoclonal antibody to KOD DNA polymerase, KOD exo(-) DNA polymerase .This product provides an antibody-mediated hot start PCR to enhance the specificity and sensitivity of PCR. This antibody inhibits polymerase activity before the onset of thermal cycling, preventing primer dimer formation and non-specific amplification. At the first denaturation step of the thermal cycling, the antibody is quickly inactivated and PCR proceeds. The antibody-mediated hot start method is significantly more convenient to use than other hot start methods.
ReverTra Ace™
ReverTra Ace™ is a high efficient M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase that has been genetically modified to remove RNase H activity and increase reaction efficiency. It is the preferred enzyme for applications requiring full-length cDNAs and high product yields from total RNA, mRNA, rRNA, etc.
RNase inhibitor
This product is a recombinant human ribonuclease inhibitor purified using a combination of ion exchange and affinity chromatography.
This inhibitor exhibits broad-spectrum RNase inhibition, including against RNase A, RNase B, RNase C, and human placental RNase but it does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, or RNase H. The 50 kDa protein exerts its inhibitory effect by noncovalently binding to RNases in a 1:1 ratio. This inhibitor can used for reverse transcriptase reactions.
Uracil DNA glycosylase
PCR amplification using non-sample DNA as a template because of contamination with the amplification product of the previous PCR is called carry-over contamination. In such cases, the negative control can also amplify target genes causing false positives or overestimation when quantitative PCRs are performed.
Carry-over contamination can be prevented by performing PCR using dNTPs containing dUTP in advance, followed by treatment with uracil-DNA glycosylase (UNG) prior to performing PCR.
Thermo T7 RNA polymerase
Thermo T7 RNA polymerase is a genetically modified T7 RNA polymerase exhibiting increased thermal stability compared with other RNA polymerases. The optimum reaction temperature of this enzyme is approximately 50°C. The half-life of the enzyme at 50°C is approximately 85 min.