Code No. CAP-101 1,000U
Calf intestine alkaline phosphatase catalyzes the removal of 5'-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. It can be used to prevent self-ligation of vectors because alkaline phosphate-treated DNA fragments lack the 5'-phosphoryl termini required by DNA ligases.
Calf intestine
One unit is defined as the amount of enzyme that will dephosphorylate 1µmole of p-Nitrophenyl phosphate with the appropriate buffer (pH 10.2) in 1 min at 37ºC.
30 mM Triethanolamine (pH7.8), 1 mM MgCl2, 50% Glycerol
Store at -20ºC
Calf Intestine Alkaline Phosphatase (1-3 U/µL)
10 x Buffer
This enzyme can be inactivated by heating at 65ºC for 30 min. However, the reacted DNA fragments should be purified by phenol-chloroform extraction or DNA fragment purification kits (e.g., MagExtractor –PCR & Clean up–, Code No. NPK-601) prior to ligation with caution.