Modifying Enzyme| Research Reagents │TOYOBO

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Modifying Enzyme

Toyobo has various modifying enzymes. The detailed information can be referred in the following linked sites.

Applicatins Product Name
High efficient reverse transcription ReverTra Ace™
Stable transcription (T7 promoter) Thermo T7 RNA polymerase
Substrate for reverse transcription dNTPs Mixture
Dephosphorylation of DNA fragments Calf intestine Alkaline Phosphatase
Prevent carryover contamination Uracil-DNA Glycosylase (UNG), Heat-labileRNase Inhibitor
/ Uracil-DNA Glycosylase (UNG), Heat-labile ＜Glycerol Free＞
cDNA synthesis, in vitro transcription, in vitro translation RNase Inhibitor

ReverTra Ace™ High Efficient Reverse Transcriptase

  • RNase minus M-MLV RTase with improved performance
  • Enables the synthesis of longer cDNAs (≥ 14 kb) than the WT-enzyme
  • Exhibits excellent reaction efficiency at high temperatures.

ReverTra Ace™ is a high efficient M-MLV (Moloney Murine Leukemia Virus) reverse transcriptase that has been genetically modified to remove RNase H activity and increase reaction efficiency. It is the preferred enzyme for applications requiring full-length cDNAs and high product yields from total RNA, mRNA, rRNA, etc.

  • Code No. TRT-101 10,000U

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Thermo T7 RNA polymerase Thermostable T7 RNA polymerase

  • Exhibits greater specific activity than WT-enzyme at 37-50ºC.

Thermo T7 RNA polymerase is a genetically modified T7 RNA polymerase exhibiting increased thermal stability. The optimum reaction temperature of this enzyme is around 50ºC. The half-life of the enzyme at 50ºC is approximately 85 min.

  • Code No. TRL-201 7,500U

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dNTPs Mixture(2mM) dNTPs Mixture(10mM)

  • 2 mM and 10 mM solutions are available
  • Suitable for PCR and reverse transcriptation

dNTPs Mixture is an equal moler solutoin mixture of ultrapure dATP, dCTP, dGTP and dTTP.

  • Code No. NTP-201 2 µmoles/1 mL (2 mM)
  • Code No. NTP-301 2 µmoles/0.2 mL (10 mM)

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Calf Intestine Alkaline Phosphatase

  • Can be inactivated by heating at 65ºC for 30 min.

Calf intestine alkaline phosphatase catalyzes the removal of 5’-phosphate groups from DNA, RNA, and ribo- and deoxyribonucleoside triphosphates. It can be used to prevent self-ligation of vectors because alkaline phosphate-treated DNA fragments lack the 5’-phosphoryl termini required by DNA ligases.

  • Code No. CAP-101 1,000U

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Uracil-DNA Glycosylase (UNG), Heat-labile
/ Uracil-DNA Glycosylase (UNG), Heat-labile ＜Glycerol Free＞

  • Useful in preventing PCR carry-over and false positives
  • Because of its high thermal sensitivity, it can be used not only for PCR but also for RT-PCR (RT-reaction should be performed at 42°C or higher)

Uracil-DNA Glycosylase (UNG) hydrolyzes the N-glycosylic bond between the deoxyribose sugar and the base in uracil-containing DNA leaving an apyrimidinic site in DNA. Since DNA with an apyrimidinic site is degraded by heat treatment, UNG can be used in combination with dUTP to prevent carry-over of amplified products derived from PCR.

  • Code No. UNG-101 200U
  • Code No. UNG-201 200U

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RNase inhibitor

This product is a recombinant type human ribonuclease inhibitor (RNase inhibitor; 51kDa) that is produced by E. coli. RNase inhibitor forms a complex with ribonuclease A (RNase A) and inhibits the activity of RNase A. This inhibitor can be applied to reverse transcriptase reactions.

  • Code No. SIN-201 2,500 U

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