Real-time PCR| Research Reagents │TOYOBO

TOYOBO Ideas & Chemistry Research Reagents

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Real-time PCR Kits

Toyobo has various real-time PCR reagents showing below. The following table shows the characteristics of each product. The detailed information can be referred in the linked sites.

Assay Type Product Name Fast cycle ＊dUTP
contain		 Passive
reference		 Specificity Efficiency Long target amplification GC-rich targets Amplification from crude samples
Probe 1-Step Hot Start TTx (RNA) Kit ✓     ++ ++ + + +++
THUNDERBIRD Probe One-step qRT-PCR kit ✓ ✓ ✓ +++ +++ + ++ ++
RNA-direct Probe Realtime PCR Master Mix     ✓ ++ ++ + + +
2-Step Hot Start TTx (DNA) Kit ✓ ✓   ++ ++ + + +++
Hot Start rTth (DNA) Kit ✓ ✓   +++ ++ + + ++
THUNDERBIRD Next Probe qPCR Mix ✓ ✓ ✓ +++ +++ + + ++
THUNDERBIRD Probe qPCR Mix     ✓ ++ ++ + + +
Realtime PCR Master Mix     ✓ + + + + +
SYBR 1-Step RNA-direct SYBR Green Realtime PCR Master Mix     ✓ ++ ++ + + +
2-Step THUNDERBIRD Next SYBR qPCR Mix ✓ ✓ ✓ +++ +++ ++ + ++
THUNDERBIRD SYBR qPCR Mix     ✓ ++ ++ + + +
KOD SYBR qPCR Mix ✓   ✓ ++ ++ +++ +++ +++
SYBR Green Realtime PCR Master Mix (-Plus-)     ✓ + + + + +

+++: BEST, ++: Excellent, +: Good, ✓: Applicable
＊Adding Uracil-N-Glycosylase(UNG) can prevent false positives due to carry-over contamination.

Hot Start TTx (RNA) Kit

  • Highly efficient one-enzyme system and 1-step qRT-PCR
  • Reverse transcription is possible at high temperatures
  • Detectability with high-speed cycles

Hot Start TTx (RNA) Kit is qRT-PCR reagent based on our original polymerase, TTx DNA Polymerase. TTx DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for 1-step qRT-PCR, including reverse transcription and PCR steps.
TTx DNA Polymerase has higher amplification efficiency than Taq DNA Polymerase, which is a general-purpose enzyme, and has reverse transcription activity, enabling amplification from a crude sample containing PCR inhibitors with high efficiency from both DNA and RNA.

  • Code No. HSTTX-111 250 reactions

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THUNDERBIRD™ Probe One-step qRT-PCR Kit High Efficient One-step qRT-PCR Kit

  • Rapid and highly sensitive
  • Tolerant of target sequence diversity
  • Tolerant of PCR inhibitors
  • Utilization of dUTP
  • Multiplex detection

THUNDERBIRD™ Probe One-step qRT-PCR Kit is a one-step real-time reverse-transcription polymerase chain reaction (RT-PCR) kit using the highly efficient reverse transcriptase "ReverTra Ace™" and Tth DNA polymerase as a PCR enzyme. This product can be used mainly in TaqMan™ probe assays. The one-step system is suitable for high-throughput analysis because of its simple reaction setup. In addition, this system can reduce the risk of cross-contamination.

  • Code No. QRZ-101 100 reactions

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RNA-direct™ Realtime PCR Master Mix One-step qPCR Master Mix for Probe Assay

  • Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
  • Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in systems using passive reference (e.g., ABI PRISM™ 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems.
  • Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible probe assay.

This product is a 2 × Master Mix for "1-step real-time PCR" using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for "1-step real-time PCR", including reverse transcription and PCR steps. This reagent is applicable for TaqMan™ assay or hybridization probe assay, in combination with each probe.

  • Code No. QRT-101 0.5 mL x 5 100 reactions

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Hot Start TTx (DNA) Kit

  • Excellent DNA Amplification Efficiency
  • Detectability with high-speed cycles
  • Tolerant of PCR Inhibitors
  • Allows multiplex PCR

Hot Start TTx (DNA) Kit is qPCR reagent based on our original polymerase, TTx DNA Polymerase. TTx DNA Polymerase has higher amplification efficiency than Taq DNA Polymerase, which is a general-purpose enzyme, and enables amplification using fast cycle condition and amplification from a crude sample containing PCR inhibitors.

  • Code No. HSTTX-101 250 reactions

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Hot Start rTth (DNA) Kit

  • Excellent DNA Amplification Efficiency
  • Tolerant of PCR Inhibitors

Hot Start rTth (DNA) Kit is PCR reagent based on Tth DNA Polymerase. Tth DNA Polymerase has higher amplification efficiency than Taq DNA Polymerase, which is a general-purpose enzyme, and enables amplification from low copies of template and amplification from a crude sample containing PCR inhibitors.

  • Code No. HSTTH-301 250 reactions

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THUNDERBIRD™ Next Probe qPCR Mix

  • Broad dynamic range
  • Fast cycles with 10 s extension times achievable
  • Improved quantitative performance in the presence of PCR inhibitors
  • Enhanced multiplex detection performance
  • Enhanced stability of prepared reaction mixtures
  • Compatibility with dUTP carryover prevention
  • Compatibility with various real-time thermocyclers and reverse transcription reagents

THUNDERBIRD™ Next Probe qPCR Mix is a 2x concentration premixed reagent for real-time PCR using various detection systems such as TaqMan™ probes and fluorescently labeled primers. The components other than ROX (provided separately), probes, and primers are pre-mixed, making the preparation of the reaction solution convenient. Additionally, this kit minimizes variation in fluorescence intensity between samples, allowing for highly reproducible results.
This product has further improved the composition of the conventional THUNDERBIRD™ Probe qPCR Mix [Code No. QPS-101], optimizing it for multiplex detection and detection from samples including PCR inhibitors. It is also compatible with high-speed PCR cycles.

  • Code No. QPX-101 500 reactions (20 μl total reaction volume)

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THUNDERBIRD™ qPCR Mix High efficient real-time PCR Master Mix

  • The specificity for the detection of low-copy targets is improved.
  • The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending).
  • High specificity and effective amplification enable the detection of a broad dynamic range.
  • The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.

THUNDERBIRD™ Probe and SYBR® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using TaqMan™ probes and SYBR® Green I. The master mix contains all required components, except for ROX reference dye, probe and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments.
These products are improved versions of Realtime PCR Master Mix (Code No. QPK-101) and SYBR® Green Realtime PCR Master Mix (Code No. QPK-201) .

  • Code No. QPS-101 200 reactions (TaqMan™ probe version)
  • Code No. QPS-201 200 reactions (SYBR® Green version)

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Realtime PCR Master Mix Efficient qPCR Master Mix for Probe Assay

  • Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible probe assay.
  • Can be used in glass capillary systems (e.g. LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in a passive reference system (e.g., ABI PRISM™7700, Applied Biosystems, Inc.).

This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer and probe. This reagent is applicable in TaqMan™ assays or hybridization probe assays, in combination with each probe.

  • Code No. QPK-101 1 mL x 5 200 reactions

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RNA-direct™ SYBR® Green Realtime PCR Master Mix
One-step qPCR Master Mix for Intercalation Assay

  • Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
  • Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in systems using passive reference (e.g., ABI PRISM™ 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems.
  • Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible intercalation assay.

This product is a 2 × Master Mix for "1-step real-time PCR" using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for "1-step real-time PCR", including reverse transcription and PCR steps. This reagent can be applied to an intercalation assay with SYBR® Green I.

  • Code No. QRT-201 0.5 mL x 5 100 reactions

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THUNDERBIRD™ Next SYBR® qPCR Mix

  • High specificity
  • Homogeneous amplification
  • Broad dynamic range
  • Compatibility for various real-time cyclers
  • Fast PCR
  • Utilization of a visible tracking dye

THUNDERBIRD™ Next SYBR® qPCR Mix is a highly efficient 2× Master Mix for real-time PCR using SYBR® Green I. The master mix contains all required components, except primers. The master mix facilitates reaction setup, and improves the reproducibility of experiments.
This product is an improved version of THUNDERBIRD™ SYBR® qPCR Mix (Code No. QPS-201). In particular, the reaction specificity and PCR efficiency is enhanced.

  • Code No. QPX-201 500 reactions

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KOD SYBR® qPCR Mix High efficient real-time PCR Master Mix with KOD

  • Quantitative analysis can be achieved using long targets, up to 2kb.
  • Because of using KOD DNA polymerase, the product is tolerant of PCR inhibitors in crude samples.
  • Enable the effective amplification from GC-rich targets.

KOD SYBR® qPCR Mix is a highly efficient 2× Master Mix for real-time PCR using SYBR® Green I and based on the 3’-5’ exonuclease deficient KOD DNA Polymerase [KOD exo(-)]. The master mix contains all the required components, except the ROX reference dye and primers (50x ROX reference dye is supplied separately with this kit). The master mix aids reaction setup, and improves the reproducibility of experiments.

  • Code No. QKD-201 200 reactions

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SYBR® Green Realtime PCR Master Mix Efficient qPCR Master Mix for Intercalation Assay

  • Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible intercalation assay.
  • Can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.).
  • Can be used in a passive reference system (e.g., ABI PRISM™ 7700, Applied Biosystems, Inc.).

This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for intercalation assay with SYBR® Green I.

  • Code No. QPK-201 1 mL x 5 200 reactions

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