GenNext™ NGS Library Prep Kit comprises the enzymes and buffers for preparing libraries for illumina^® sequencing from fragmented double-stranded DNA and PCR products. With this system, it is possible to conveniently and quickly convert a broad range (1ng - 1μg) of input amounts of DNA into libraries for illumina^® sequencing. Terminal repair and 3' end adenylation of the fragmented DNA can be conducted in the end repair and A-tailing step. Platform-specific adapters are then ligated to both ends of the DNA fragments.
If required, a high-fidelity amplification step can be performed using the reagents included in the GenNext™ NGS Library Prep Kit. Library Amplification Master Mix uses a highly-accurate PCR enzyme developed using genetically-modified KOD DNA polymerase. This minimizes the influence of GC bias on amplification and can amplify various regions evenly.

GenNext™ NGS Library Quantification Kit is for the SYBR^® Green I qPCR-based library quantification of Illumina next-generation sequences. The kit allows the specific and accurate quantification of libraries bearing P5 and P7 adaptors which can be applied to flow cell amplification. It uses the highly efficient qPCR master mix KOD SYBR^® qPCR Mix.

GenNext™ RamDA-seq™ Single Cell Kit is a kit using RT-RamDA™ (Reverse Transcription with Random Displacement Amplification) method for preparing cDNA for NGS analysis from single-cell and Ultra-low amount RNA.
RT-RamDA™ is a novel cDNA amplification method utilizing the strand displacement activity of reverse-tr​anscriptas​e. GenNext™ RamDA-seq™ Single Cell Kit can detect not only poly(A) RNA but also non-poly(A) with high sensitively by using random primer or NSR primer*, allowing the detection of many genes compared with the conventional method.
Unlike conventional method using Oligo-dT primer, cDNA for full-length total RNA-seq can be synthesized by RT-RamDA™ method.
In addition, because cDNA is amplified at the same time as it is synthesized, RamDA-seq™ method does not require amplification adaptors and PCR amplification step, and it reduces the biases caused by PCR amplification.

^*NSR primer is abbreviation for Not so random primer.
^*RamDA-seq™ and RT-RamDA™ are a trademark of RIKEN, Institute of Physical and Chemical Research.

GenNext™ Shin-RamDA-seq™ Single Cell Stranded Kit is an improved version of the GenNext™ RamDA-seq™ Sigle Cell Kit, and is a kit for preparing libraries for next-generation sequencing (NGS) from single cell or trace RNA. Obtained libraries could be applied to subsequent strand specific RNA-seq analysis. By using this kit, you can prepare NGS libraries that cover the full length of RNA and also perform strand analysis.
This is a kit using RT-RamDA™ (Reverse Transcription with Random Displacement Amplification) method developed by RIKEN. RT-RamDA™ is a novel cDNA amplification method utilizing the strand displacement activity of reverse-tr​anscriptas​e. This kit can detect not only poly(A) RNA but also non-poly(A) with high sensitively by using random primer or NSR primer^*. Additionally, Shin-RamDA-seq™ incorporates further reduction of rRNA and preservation of strand information, enabling more accurate RNA-Seq analysis.

^*NSR primer is abbreviation for Not so random primer.
^*Shin-RamDA-seq™ is an abbreviation for "Stranded High-Sensitivity Random Displacement Amplification sequencing".
^*Shin-RamDA-seq™, RamDA-seq™ and RT-RamDA™ are a trademark of RIKEN, Institute of Physical and Chemical Research