ReverTra Ace-α-™ is an efficient and convenient kit to synthesize high quality cDNA. This kit contains the highly efficient reverse transcriptase "ReverTra Ace™", as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR. ReverTra Ace™ is an M-MLV reverse transcriptase that has been improved by point mutation technology. ReverTra Ace™ has two mutations in the polymerase and RNase H domains.

The ReverTra Ace™ qPCR RT Kit is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR. This kit contains the highly efficient reverse transcriptase "ReverTra Ace™" and a RT buffer optimized for the highly efficient synthesis of short-chain cDNAs suitable for real-time PCR. The protocol is simple, and the reaction can be completed in 15 min. ReverTra Ace™ is a mutant-type M-MLV reverse transcriptase shows excellent efficiency.
ReverTra Ace™ qPCR RT Master Mix (Code No. FSQ-201) is a premix version of ReverTra Ace™ qPCR RT Kit. The master mix reagent (5x) contains the highly efficient reverse transcriptase "ReverTra Ace™" , primers and buffer optimized for highly efficient synthesis of short-chain cDNAs suitable for real-time PCR.

This kit includes master mix reagents for reverse transcription and for the removal of genomic DNA [DNase I treatment]. In many cases, total RNA prepared using spin-columns or acid guanidium-phenol- chloroform (AGPC) extraction methods contains small amount of genomic DNA. Any contaminating genomic DNA will be amplified along with cDNA, especially when primer pairs are designed within the same exon or from pseudogenes. Amplification from genomic DNA can result in qualitative and quantitative inaccuracies. The genomic DNA degradation and revers transcription steps can be achieved sequentially without purification or heat inactivation of DNase I.

SuperPrep™ Cell Lysis & RT Kit for qPCR (Code No. SCQ-101) consists of "Lysis Reagents" and "RT Reagents" for synthesis of cDNA templates for real-time PCR assays. "Lysis Reagents" can be used to prepare cell lysates containing RNAs that can be used as templates for reverse transcription.
"RT Reagents" can be used to carry out reverse transcription, and are optimized for efficient cDNA Synthesis from crude lysates. The synthesized cDNA can be used as a template for two step real-time PCR. The cell lysate prepared by "Lysis Reagents" can be applied to one-step real-time PCR.

SuperPrep™ II Cell Lysis & RT Kit for qPCR (Code No. SCQ-401) consists of "Lysis Reagents" and "RT Reagents" for synthesis of cDNA templates for real-time PCR assays.
"Lysis Reagents" prepares cell lysates containing RNAs that can be used as templates for reverse transcription. "RT Reagents" contains reagents for reverse transcription, optimized for efficient cDNA Synthesis from crude lysates. The synthesized cDNA can be applied to real-time PCR directly. This assay system is suitable for high-throughput assays. In the previous version, it was necessary to add Stop Solution after adding Lysis Solution. In SuperPrep® II, cell lysates with lysis solution can be used as templates for cDNA Synthesis directly.

miRNAssay™ qPCR RT Master Mix [Code No. MIR-101] is a real-time PCR reverse transcription reagent for miRNA developed using the high-efficiency reverse transcriptase ReverTra Ace™. It is optimized for reverse transcription reactions using stem-loop RT primers and can synthesize cDNA from miRNA with high specificity and sensitivity.
SuperPrep™ miRNAssay™ Cell Lysis & RT Kit for qPCR [Code No. MIR-201] consists of the above miRNA-specific reverse transcription master mix and lysis reagents for cultured cells and exosomes. This kit enables high-quality cDNA synthesis from cultured cells or recovered exosomes without purification of RNA.

The QuantAccuracy™, RT-RamDA™ cDNA Synthesis Kit (Code No. RMQ-101) is an efficient and convenient kit to synthesize cDNA from single cells or trace amounts of RNA for real-time PCR analysis.
By using this kit, cDNA covering full-length total RNA can be synthesized, and gene expression analysis can be performed with high sensitivity.
*RT-RamDA™ and RamDA-seq™are trademarks of RIKEN, Institute of Physical and Chemical Research, Japan