Immunoreaction Enhancer Solution
Code No. NKB-401 5 mL x 2 / NKB-501 20 mL x 1 / NKB-601 20 mL x 1
Can Get Signal™ immunostain is a reaction solution that contains an accelerator for antigen-antibody reactions, which improves sensitivity, specificity, and S/N of immunohistochemistry (IHC) and immunocytochemistry.
Store at 4ºC
This kit includes the following components. All reagents should be stored at 4ºC and protected from light.
Reagent Name | Code No. | ||
---|---|---|---|
NKB-401 | NKB-501 | NKB-601 | |
Solution A | 5 mL | 20 mL | - |
Solution B | 5 mL | - | 20 mL |
Flow chart of immunostaining with Can Get Signal™ immunostain
The localization of PCNA (proliferating cell nuclear antigen) expression in human skin was detected using paraffin-embedded sections of the human skin tissue model TESTSKINTM (Toyobo). Detection was performed by the ABC method with anti-PCNA mouse monoclonal antibody as the primary antibody and biotinylated mouse IgG as the secondary antibody. Each antibody was diluted with Solution A of Can Get Signal™ immunostain prior to use. As a control experiment, PBS(-) containing 1.5% normal horse serum (conventional method) was used instead of Solution A of Can Get Signal™ immunostain. As a result, Can Get Signal™ immunostain produced higher signals and lower background than the conventional method.
The localization of paxillin in Swiss 3T3 cells was detected using anti-paxillin polyclonal antibody and Alexa488-conjugated rabbit IgG antibody. As a result, the exposure time for detection could be reduced from 3 s to 1 s by using Can Get Signal™ immunostain, and the detailed fiber structure (fibrillar adhesion) could also be detected by using Can Get Signal™ immunostain. However, the structure could not be detected with the conventional method due to high background signals.
Fig. 2C shows a merged picture of the immunological detection with anti-paxillin and anti-phospho-tyrosine antibodies, and actin staining.
*The data was kindly provided by Dr. Harada, Tokyo Institute of Technology.