• MANUAL：QPS-101
• MANUAL：QPS-201
• SDS：QPS-101
• SDS：QPS-201

DESCRIPTION

• THUNDERBIRD™ Probe and SYBR^® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using TaqMan™ probes and SYBR^® Green I. The master mix contains all required components, except for ROX reference dye, probe and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments.
  These products are improved versions of Realtime PCR Master Mix (Code No. QPK-101) and Realtime PCR Master Mix (Code No. QPK-201) . In particular, reaction specificity and PCR efficiency is enhanced.

  This product is sold in U.S. under the license of US patent 7772383
  from Chakrabarti Advanced Technology NewCo LLC.

• Features
• Details
• Application Data

Features Details Application Data

Features

• ・The specificity for the detection of low-copy targets is improved.
• ・The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending)
• ・High specificity and effective amplification enable the detection of a broad dynamic range.
• ・The reagent is applicable to most real-time cyclers (i.e. Block type and glass capillary type). Because the 50x ROX reference dye is individually supplied with this kit, the kit can be applied to real-time cyclers that require a passive reference dye.
• ・The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.

Details

Storage condition

Store at -20ºC

Components

<QPS-101T>

THUNDERBIRD™ Probe qPCR Mix	1 mL × 1
50x ROX reference dye	50 µL × 1

<QPS-101>

THUNDERBIRD™ Probe qPCR Mix	1.67 mL × 3
50x ROX reference dye	250 µL × 1

<QPS-201T>

THUNDERBIRD™ SYBR® qPCR Mix	1 mL × 1
50x ROX reference dye	50 µL × 1

QPS-201

THUNDERBIRD™ SYBR® qPCR Mix	1.67 mL × 3
50x ROX reference dye	250 µL × 1

Compatible Real-Time Instruments

Applied Biosystems	ABI PRISM™ 7000	Roche Diagnostics	LightCycler™ 1.x / 2.0
	ABI PRISM™ 7700		LightCycler™ Nano
	Applied Biosystems™ 7300		LightCycler™ 480
	Applied Biosystems™ 7500/7500FAST	Bio-Rad / MJ	MiniOpticon™
	Applied Biosystems™ 7900HT		CFX96 Touch™
	Applied Biosystems™ StepOne™	Agilent Technologies	Mx3000P / Mx3005P / Mx4000
	Applied Biosystems™ StepOnePlus™	TaKaRa	Thermal Cycler Dice™ Real Time Systems

Application Data

Example 1. Comparison of the sensitivity and efficiency of the SYBR^® Green I assay

β-actin mRNA was detected with serially diluted cDNA from HeLa cell total RNA. THUNDERBIRD™ SYBR^® qPCR Mix [Code No. QPS-201] showed greater sensitivity and efficiency than other kits (companies A and B).

Example 2. Comparison of the PCR efficiency

Norovirus G1 and human GAPDH genes were detected using serially diluted cDNA samples by SYBR^® Green I and TaqMan real-time PCR. THUNDERBIRD™ qPCR Master Mix showed greater efficiency than other reagents.

Detection of Norovirus G1 gene
by SYBR^® Green I assay.
[Roche Diagnostics LightCycler 1.1]

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Detection of GAPDH gene
by TaqMan™ assay.
[Roche Diagnostics LightCycler 1.1]

Example 3. Verification of the measurement accuracy

Human GAPDH genes were detected using serially 20.5 fold diluted cDNA synthesised from HeLa cell total RNA by SYBR^® Green I assay. THUNDERBIRD™ qPCR Master Mix successfully detected the differences between dilutions.

Detection of human GAPDH gene by SYBR^® GREEN I assay.
[Applied Biosystems 7900HT]