- HOME
- A simple and high-yield exosome isolation kit CATAROSEVTM
-
A simple and high-yield exosome isolation kit
CATAROSEVTM
Code No. CTS-001T, CTS-001
DESCRIPTION
CATAROSEV™ is a product that uses a precision microfiltration membrane developed by Toyobo, which possesses both ion‑exchange functionality and size‑based separation capability. By leveraging the size and charge characteristics of small EVs, it enables their efficient, high‑purity separation and recovery in a short amount of time.
Features
- Easy‑to‑use
Complicated pretreatment is not required, and small EVs can be recovered in approximately 30 minutes.
- High yield
Enables the recovery of ~10¹⁰ small EVs from culture supernatants, serum, fruit juices, and various other biological samples.
- High selectivity
Achieves high‑efficiency recovery of 50–150 nm particles in an intact, undamaged condition.
※ small EVs: extracellular vesicles enriched in exosomes
Details
STORAGE CONDITION
Store at 4°C
COMPONENTS
The kits include the following reagents.
| Exosome Isolation Kit CATAROSEV™ | CTS-001T | CTS-001 |
|---|---|---|
| Syringe Filter (Ion Exchange menbrane) | 1 set | 5 set |
| Three-way stopcock | 1 set | 5 set |
We also offer a buffer set (for 5 purifications) sold separately. Please purchase it if necessary.
| Product | Code No. | Volume |
|---|---|---|
| Buffer Set for CATAROSEV™ | CTS-011 | Salt concentration adjustment buffer 1 x 100mL Equilibration/washing buffer 1 x 100mL Elution buffer 1 x 100mL |
Workflow
Adsorption Step : small EVs are adsorbed onto the membrane, using the charges on the ssmall EVs surface.
Wash Step : Uncharged impurities are removed.
Elution Step : small EVs that are adsorbed to the membrane are collected using a high-salt buffer.
Mechanism of CATAROSEV™
Application Data
Example 1.Particle size comparison
Extracellular vesicles were isolated from HEK cell culture supernatants using CATAROSEV™ and ultracentrifugation, followed by particle size analysis with a NanoSight NS300.
Compared with ultracentrifugation, CATAROSEV™ captured fewer large particles and more efficiently isolated 50‒150 nm particles enriched in small EVs.
Example 2.Comparison of EV recovery rates from HEK supernatants and serum
Extracellular vesicles were recovered from 10 mL of HEK cell culture supernatants or 200 μL of serum aliquots using CATAROSEV™ or ultracentrifugation, and particle size, recovery rates, and CD63/CD9 protein yields were compared.
CATAROSEV™ showed higher recovery yields.
