Protein research

The detailed information can be referred in the following linked sites.

Applications Product Name
Enhancement of Western blot & ELISA Can Get Signal™
Efficient Blocking Buffer PVDF Blocking Reagent for Can Get Signal™
Enhancement of IHC Can Get Signal™ immunostain

Enhancement of Western blot & ELISA

Can Get Signal™ Immunoreaction Enhancer Solution

  • Enhances immunoassay signals up to several dozen times by maintaining low background signals.
  • Can be used in combination with secondary antibodies labeled with peroxidase or alkaline phosphatase, etc.
  • Can be used directly without dilution (ready-to-use).

Can Get Signal™ is a solution containing an accelerator for antigen-antibody reactions. This reagent improves sensitivity, specificity, and signal-to-noise ration (S/N) for Western blotting, dot blotting, enzyme-linked immunosorbent assay (ELISA), etc. Solutions 1 and 2 refer to the reactions of the primary and secondary antibodies, respectively.

  • Code No. NKB-101T 50 mL x 2
  • Code No. NKB-101 250 mL x 2
  • Code No. NKB-201 250 mL x 1
  • Code No. NKB-301 250 mL x 1

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Efficient Blocking Buffer

PVDF Blocking Reagent for Can Get Signal™ Blocking reagent for western blot analysis

  • Optimized for use together with Can Get Signal™ Immunoreaction Enhancer Solution (Code No.NKB-101) for western blot analysis.
  • Suitable for detection of phosphorylated proteins because it does not contain any protein components.
  • Unlike conventional blocking reagents (e.g., non-fat milk and gelatin), minimize the masking effects of low signal intensities.

PVDF Blocking Reagent for Can Get Signal™ is a high performance blocking reagent optimized for western blot analysis. The reagent consists of a synthesized polymer, with no protein components. The reagent can be used efficiently with Can Get Signal

*This reagent is manufactured by NOF CORPORATION.

  • Code No. NYPBR01 500 mL

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Enhancement of IHC

Can Get Signal™immunostain Immunoreaction Enhancer Solution

  • Improves sensitivity, specificity, and S/N of IHC.
  • Can be applied to various detection systems (e.g., chromogenic, chemiluminescence, or fluorescence).
  • Can be used with ABC or polymer complex methods.
  • Solutions A and B exhibit various properties for improving results.
  • Can be used directly without dilution (Ready-to-use).

Can Get Signal™ immunostain is a reaction solution that contains an accelerator for antigen-antibody reactions, which improves sensitivity, specificity, and S/N of immunohistochemistry (IHC) and immunocytochemistry.

  • Code No. NKB-401 5 mL x 2
  • Code No. NKB-501 20 mL x 1
  • Code No. NKB-601 20 mL x 1

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