THERMOSTABLE T7 RNA POLYMERASE

Fig. 1. Comparison of the transcripts with wild-type T7 RNA polymerase.

Thermo T7 RNA polymerase (Code No. TRL-201) is genetically engineered T7 RNA polymerase exhibiting increased thermal stability. The optimum reaction temperature of this enzyme is around 50ºC, and the half-life at 50ºC of this enzyme is approximately 85 min, whereas that of wild-type T7 RNA polymerase is approximately 1.9 min.
This enzyme is useful for the transcription at higher temperatures in various applications.

  • Fig. 2. omparison of the specific activity with wild-type T7 RNA polymerase.

  • Fig. 3. Comparison of the half-life with wild-type T7 RNA polymerase.