FAQs

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KOD FX

What type of ends does KOD FX leave?
KOD FX will leave blunt-ended products only.
What is the error rate of KOD FX?
The error rate of KOD FX is about 10 times lower than that of rTaq DNA polymerase.

KOD -Plus-

What type of ends does KOD -Plus- leave?
KOD -Plus- will leave blunt-ended products only.
What is the error rate of KOD-Plus?
KOD -Plus- and KOD -Plus- Ver. 2 are the highest fidelity thermostable polymerases. Their error rate is 83 times lower than that of rTaq DNA polymerase.

Blend Taq/Blend Taq-Plus

What type of ends does Blend Taq leave?
Blend Taq will leave dA-tailed products.
What is the error rate of Blend Taq?
The error rate of Blend Taq and Blend Taq -Plus is about 4 times lower than that of rTaq DNA polymerase.
How is Blend Taq -Plus- different from Blend Taq?
Anti-Taq antibody is added to Blend Taq -Plus- to allow for hot-start PCR.

KOD Dash

What type of ends does KOD Dash leave?
KOD Dash will leave dA-tailed products.
What is the error rate of KOD Dash?
The error rate of KOD Dash is about 4 times lower than that of rTaq DNA polymerase.

rTth DNA polymerase

What type of ends does rTth DNA polymerase leave?
rTth DNA polymerase will leave dA-tailed products.
What is the error rate of rTth DNA polymerase?
The error rate of rTth DNA polymerase is the same as that of rTaq DNA polymerase.

rTaq DNA polymerase

What type of ends does rTaq DNA polymerase leave?
rTaq DNA polymerase will leave dA-tailed products.

Anti-Taq high

Will Anti-Taq high work with polymerases other than Taq?
Anti-Taq high should work with all Taq variants, but not with KOD.

Real-time PCR Master Mix

Which Real-time PCR Master Mix protocol should be used with particular instruments?
Real-time PCR Master Mix manuals often outline protocols adapted for ABI 7700, ABI 7900, Roche LightCycler, and BioFlux LineGene.

RNA-direct Real-time PCR Master Mix

Which Real-time PCR Master Mix protocol should be used with particular instruments?
Real-time PCR Master Mix manuals often outline protocols adapted for ABI 7700, ABI 7900, Roche LightCycler, and BioFlux LineGene.

MagExtractor -Genome-

What is the size of genomic DNA that is obtained with MagExtractor -Genome-?
Genomic DNA purified using MagExtractor -Genome- has an average length of 40 kb.
From which types of samples can DNA be isolated?
DNA can be obtained from all types of solid tissues, tissue cultured cells, and blood.
What is the expected yield?
The yield varies depending upon the sample type from which DNA is extracted. Typical yields obtained are: 5 µg/5 mg tissue, 3 µg/5 x 105 cultured cells and 2 µg/100 µL whole blood.

MagExtractor -RNA-

From which types of samples can total RNA be isolated?
Total RNA can be obtained from all types of solid tissues, tissue cultured cells, and yeasts.
What is the expected yield?
This varies depending upon the sample type from which total RNA is extracted. Typical yields obtained are: 15 µg/30 mg tissue, 10 µg/1 x 106 cultured cells, and 20 µg/5 O.D. (660 nm) yeasts.

MagExtractor -Plasmid-

What is the expected yield?
The typical yield is 3-6 µg/3-9 O.D. (pUC18/JM109).

MagExtractor -Viral RNA-

From which types of samples can RNA be isolated?
RNA can be obtained from HCV, HGV, and HIV.

MagExtractor -Plant Genome-

What is the expected yield?
The typical yield is 3-5 µg/100 mg leaf.

KOD -Plus- Mutagenesis Kit

What are the longest templates tested for mutation?
We tested templates as long as an 11.3-kb plasmid.
What is the error rate of KOD -Plus-?
KOD -Plus- is the highest fidelity thermostable polymerase. Its error rate is 83 times lower than that of rTaq DNA polymerase.